We narrowed to 24,716 results for: Nov

1. pAS_4xMma PylT_FLAG-AcKRS

   Plasmid

   #140021

   Purpose

   Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AcKRS-3 (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L301M, L305I, Y306F, L309A, C348F

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

   Plasmid

   #140018

   Purpose

   Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   EcoTyrRS
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y37L, D182S, F183M, L186A

   Promoter

   EF1

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mKate2-P2A-APEX2-2xFYVE_hrs

   Plasmid

   #67663

   Purpose

   Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-2xFYVE
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

   Plasmid

   #174899

   Purpose

   Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mKate2-P2A-APEX2-OSBP

   Plasmid

   #67664

   Purpose

   Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-OSBP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-h-mmPtch1-B-HA-TagBFP

   Plasmid

   #120910

   Purpose

   Expresses BFP tagged Ptch1-B

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBetaActin-FKBP-mScarlet-Dync1h1motor

   Plasmid

   #191330

   Purpose

   Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
   

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry

   Plasmid

   #71282

   Purpose

   Zebrafish expression vector for heat-shock inducible expression of APEX2-GBP

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2-GBP
   

   Use

   Zebrafish expression with tol2

   Promoter

   HSP70l

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mKate2-P2A-APEX2-ML1N*2

   Plasmid

   #67665

   Purpose

   Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-ML1N*2
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-STAM1

    Plasmid

    #164628

    Purpose

    Expresses STAM1 in mammalian cells with a GFP tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    STAM1 (STAM Human)
    

    Tags

    cycle 3 GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. KIF5C(1-560)-2xmCherry-EF(C)

    Plasmid

    #61664

    Purpose

    Tandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)
    

    Expression

    Mammalian

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-CBX1mut-PA-mCherry

    Plasmid

    #138251

    Purpose

    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

    Depositor

    Jin Zhang

    Article

    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

    Insert

    CBX1mut-PAmCherry
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB80-hsKIF5B(1-560)-L-GFP

    Plasmid

    #193716

    Purpose

    Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)-L-GFP (KIF5B Synthetic, Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560);

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pODN-AAVS1-mNG-CAAX

    Plasmid

    #183870

    Purpose

    Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-CAAX

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Promoter

    CMV

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-HypaCas9-RHOA_sgRNA

    Plasmid

    #183878

    Purpose

    pX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    RHOA sgRNA spacer (RHOA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHS1928

    Plasmid

    #205967

    Purpose

    JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH all proteins, with His-tagged Cas8
    

    Tags

    14xHis on Cas8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS1861

    Plasmid

    #205974

    Purpose

    FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH
    

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKM50

    Plasmid

    #123656

    Purpose

    Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cells

    Depositor

    Kevin Maringer

    Article

    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

    Insert

    Aedes aegypti UbL40 promoter
    

    Use

    Luciferase

    Expression

    Insect

    Promoter

    UbL40 promoter

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pODN-H2BC11-mR2

    Plasmid

    #183866

    Purpose

    Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 homology arms with linker-mRuby2 (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mRuby2

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pODN-mR2-MYH10

    Plasmid

    #183869

    Purpose

    Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 homology arms with mRuby2-linker (MYH10 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mRuby2-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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