We narrowed to 2,503 results for: gem

1. pAAV-Ef1a-DIO ChETA-EYFP

   Plasmid

   #26968

   Purpose

   AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T, H134R

   Promoter

   EF1alpha

   Available since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. BII-Sh-FUCCI-Luc

   Plasmid

   #133364

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Use

   Tags

   HA-tagged Clover-Geminin

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-SEP-GluR2(Q)

   Plasmid

   #24002

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR2 (Gria2 Rat)
   

   Use

   Tags

   GluR2 signal peptide (aa1-24) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   R607Q

   Promoter

   CMV

   Available since

   April 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. TetOn-REEP5-mCherry-eGFP

   Plasmid

   #139870

   Purpose

   Dox-inducible lentiviral expression of human REEP5

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   REEP5 (REEP5 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-X1-Neo-GFP-ATL3

   Plasmid

   #109024

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL3 (ATL3 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EIF-1a promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV-EF1amCherry-2A-OsTIR1-W

   Plasmid

   #159296

   Purpose

   Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   OsTIR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1a promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCit-4aa-SNAP25B

   Plasmid

   #53235

   Purpose

   Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   SNAP-25B (Snap25 Rat)
   

   Use

   Tags

   4aa-linker and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1-alpha-IIb

   Plasmid

   #27288

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin alpha IIb (ITGA2B Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_NFL(WT)-FLAG

   Plasmid

   #182654

   Purpose

   Expresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   wild type mouse neurofilament light chain (Nefl Mouse)
   

   Use

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. M13cp-dg2 hp

    Plasmid

    #218094

    Purpose

    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pII

    Depositor

    Kevin Esvelt

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    GAL4-responsive UAS hsp70

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV-EF1amCherry-W

    Plasmid

    #159295

    Purpose

    Lentiviral vector expressing mCherry driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCer-4aa-VAMP2

    Plasmid

    #53233

    Purpose

    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pLenti-CaMKIIa-VChR1-EYFP-WPRE

    Plasmid

    #20954

    Purpose

    3rd gen lentiviral expression of VChR1 fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

    Insert

    volvox channelrhodopsin-1
    

    Use

    Lentiviral

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-X1-Neo-HA-SEC62

    Plasmid

    #139865

    Purpose

    Lentiviral expression of HA-tagged human SEC62

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    SEC62 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-X1-Neo-GFP-ATL2

    Plasmid

    #109020

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2 (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

    Plasmid

    #63167

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-cMyc"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

    Plasmid

    #63169

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-FLAG"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-HA-UFM1-dCS

    Plasmid

    #139869

    Purpose

    Mammalian expression of HA-tagged human UFM1-cCS

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    UFM1 (UFM1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    d84-85

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-X1-Neo-TEX264-HA

    Plasmid

    #139866

    Purpose

    Lentiviral expression of HA tagged (C terminal) human TEX264

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge