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We narrowed to 2,432 results for: gem

Showing: 1101 - 1120 of 2432 results

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pFKBPmCherry-Akt2(K14A)

Plasmid

#86597

Purpose

Expresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

FKBB-mCherry-Akt2(K14A) (AKT2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)

Plasmid

#86595

Purpose

Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E) (AKT2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVA.008

Plasmid

#22841

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA (with MCS)

Use

Cotton vigs vector

Tags

Expression

Bacterial

Mutation

Coat Protein (AR1 gene) of Cotton Leaf Crumple Vi…

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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mCer-Vamp2(qv/fw)

Plasmid

#53238

Purpose

Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

VAMP2 (Vamp2 Rat)

Use

Tags

4aa-linker and mCerulean

Expression

Mammalian

Mutation

Mutated Q76 to V; F77 to W.

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-Akt2(T309A)

Plasmid

#86625

Purpose

Expresses eGFP-tagged human Akt2(T309A) phosphomutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(T309A) (AKT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKmt2d-sg3

Plasmid

#192167

Purpose

Lenti-sgKmt2d-sg3

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVB1.3

Plasmid

#22844

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

B DNA

Use

Cotton vigs vector

Tags

Expression

Bacterial

Mutation

1.3 unit B componet length inserted into pBlueScr…

Promoter

Available since

March 16, 2010

Availability

Academic Institutions and Nonprofits only

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pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

Plasmid

#63170

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-OLLAS"

Use

Tags

N-myristoylation

Expression

Insect

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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BII-Sh-FUCCI-Luc

Plasmid

#133364

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Clover-Geminin-IRES-mKO2-Cdt1

Use

Tags

HA-tagged Clover-Geminin

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D_dC

Plasmid

#176466

Purpose

express VPS13D lacking C-terminal 407 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Use

Tags

GFP-Ub

Expression

Mammalian

Mutation

C-terminal 407 aa is deleted.

Promoter

CMV

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pESUMO-hRORg2

Plasmid

#170329

Purpose

Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Article

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 2 (RORC Human)

Use

Tags

HisSUMO

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL3664

Plasmid

#162240

Purpose

Transcribes E. coli SRP RNA sequence for SHAPE-Seq

Depositor

Julius Lucks

Article

Yu et al Mol Cell. 2021 Feb 18;81(4):870-883.e10.

Inserts

E. coli SRP RNA
HDV

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-Akt2(K14A)

Plasmid

#86624

Purpose

Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A) (AKT2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-NDUFB4

Plasmid

#139840

Purpose

Lentiviral expression of human NDUFB4

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

NDUFB4 (NDUFB4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

Plasmid

#52878

Purpose

Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

Depositor

Thomas Oertner

Article

Wietek et al Science. 2014 Mar 27.

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E90R, D156N, T159C

Promoter

CaMKIIa

Available since

July 16, 2014

Availability

Academic Institutions and Nonprofits only

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pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate

Plasmid

#238372

Purpose

Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nmKate

Use

Tags

Expression

Mutation

Promoter

cmlc2 (myl7)

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-Spe-KpnI-ubb-nmKate

Plasmid

#238373

Purpose

Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nmKate

Use

Tags

Expression

Mutation

Promoter

ubiquitin

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA-C63S-C66S

Plasmid

#225082

Purpose

Expresses precursor peptide ChrA-C63S/C66S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C63S/C66S

Use

Tags

His6

Expression

Bacterial

Mutation

C63S/C66S

Promoter

T7

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA-C66S

Plasmid

#225081

Purpose

Expresses precursor peptide ChrA-C66S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C66S

Use

Tags

His6

Expression

Bacterial

Mutation

C66S

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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