We narrowed to 2,567 results for: gem

1. pCas27

   Plasmid

   #82384

   Purpose

   sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23117

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas15

   Plasmid

   #82383

   Purpose

   sgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23119

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas14

   Plasmid

   #82382

   Purpose

   sgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23119

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas13

   Plasmid

   #82381

   Purpose

   sgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23119

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBP-J23108

   Plasmid

   #72964

   Purpose

   Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23108 promoter
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBP-J23114

   Plasmid

   #72965

   Purpose

   Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23114 promoter
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNP3614-MM168 [pNP3613 - ISCro4 Recombinase (S30T/P54Q/S243H)]

   Plasmid

   #247266

   Purpose

   Express ISCro4 recombinase

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Insert

   pNP3613 - ISCro4 Recombinase (S30T/P54Q/S243H)
   

   Expression

   Mammalian

   Mutation

   S30T/P54Q/S243H

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIB-HIS-TEV-scCAK1

   Plasmid

   #177014

   Purpose

   Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
   

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-beta-3

   Plasmid

   #27289

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin beta 3 (ITGB3 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNP3440 [pNP2922 - ISCro4 bRNA (TBL2, WT + DBL1, WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247263

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA1-179 (WT, TBL2 + DBL1)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNP2389 [pNP2314 - ISCro4 Donor + ISCro4 Target-RTG-Matched]

    Plasmid

    #247251

    Purpose

    Measure ISCro4 activity via luciferase expression

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2314 - ISCro4_Donor, ISCro4_Target_RTG-Matched
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNP2390 [pNP2322 - ISCro4 Donor + ISCro4 Target-RTG-Matched]

    Plasmid

    #247252

    Purpose

    Measure ISCro4 activity via mCherry expression

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2322 - ISCro4_Donor, ISCro4_Target_RTG-Matched
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. M13cp-dg3 hp

    Plasmid

    #218095

    Purpose

    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

    Depositor

    Kevin Esvelt

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-X1-Neo-HA-SEC62

    Plasmid

    #139865

    Purpose

    Lentiviral expression of HA-tagged human SEC62

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    SEC62 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-Akt1(WT)

    Plasmid

    #86637

    Purpose

    Expresses eGFP-tagged human Akt1(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Expression

    Mammalian

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV-EF1amCherry-2A-OsTIR1-W

    Plasmid

    #159296

    Purpose

    Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    OsTIR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    human EF1a promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-PH(Akt2)

    Plasmid

    #86596

    Purpose

    Expresses mCherry-tagged PH(WT) domain of human Akt2

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt2 PH domain (AKT2 Human)
    

    Expression

    Mammalian

    Mutation

    Amino acids 1 - 108

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-SEP-GluR1

    Plasmid

    #24000

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR1 (Gria1 Rat)
    

    Tags

    GluR1 signal peptide (aa1-22) and SEP (supereclip…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF1-alpha-IIb

    Plasmid

    #27288

    Depositor

    Timothy Springer

    Article

    Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

    Insert

    integrin alpha IIb (ITGA2B Human)
    

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-X1-Neo-GFP-ATL2

    Plasmid

    #109020

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2 (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    EIF-1a promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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