We narrowed to 1,208 results for: maz

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

Plasmid

#67976

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmAG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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P521_VSX2_p2A-h2b_mRuby3

Plasmid

#239104

Purpose

Used in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 gene

Depositor

Karl Wahlin

Article

Jones et al Cells. 2022 Oct 28;11(21):3412. doi: 10.

Insert

VSX2 (VSX2 Human)

Use

CRISPR

Tags

-p2A-h2b-mRuby3

Expression

Mammalian

Promoter

Endogenous VSX2 promoter

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

Plasmid

#202762

Purpose

Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

NEUROG2 (NEUROG2 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

Plasmid

#67975

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AZsG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

Plasmid

#67984

Purpose

Cas9 activity reporter with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

Plasmid

#67990

Purpose

CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pNTK human MBD3 PGK-Neo-pA flox

Plasmid

#52362

Purpose

Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

MBD3 (MBD3 Human)

Use

Targeting

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-Flag-Mbd3 delta1-70

Plasmid

#52372

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

deleted amino acids 1-70

Promoter

Ubiquitin

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-Flag-Mbd3 delta 25-83

Plasmid

#52373

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

amino acids 25-83 deleted

Promoter

Ubiquitin

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-Flag-Mbd3 delta49-63

Plasmid

#52374

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

deleted amino acids 49-63

Promoter

Ubiquitin

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #2

Plasmid

#61514

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gccgctcccggatctcctgc

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

Plasmid

#67983

Purpose

Cas9 activity reporter (control) with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5A tail only

Plasmid

#166956

Purpose

Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A (820-1027aa)

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBRPy CAGGS-Mbd3-IRES-PURO

Plasmid

#52376

Purpose

Mammalian expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Tags

2xFlag (DYKDDK)

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #1

Plasmid

#61515

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gcggcagctcctagctcagc

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-KIF5A-HA-IRES-Puro

Plasmid

#166952

Purpose

Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Use

Lentiviral

Tags

HA

Promoter

eF1a

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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mCitrine-DNAJC5 delL116

Plasmid

#246734

Purpose

Mammalian expression of human DNAJC5 L116 deleted with a mCitrine (YFP) tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

mCitrine

Expression

Mammalian

Mutation

delL116

Promoter

CMV

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX GST-tev-hDNAJC5

Plasmid

#246743

Purpose

Bacterial expression of human DNAJC5 wildtype with GST and a cleavage site for TEV protease on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

GST

Expression

Bacterial

Promoter

lacZ

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX GST-tev-hDNAJC5 L115R

Plasmid

#246745

Purpose

Bacterial expression of human DNAJC5 L115R with GST and a cleavage site for TEV protease on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

GST

Expression

Bacterial

Mutation

L115R

Promoter

lacZ

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6 FLAG-DNAJC5 L115R

Plasmid

#246742

Purpose

Mammalian expression of human DNAJC5 with L115R mutation, and a FLAG tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L115R

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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