We narrowed to 3,175 results for: tef

Showing: 1101 - 1120 of 3175 results

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pNC952

Plasmid

#20401

Depositor

Beverly Errede

Publication

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP-reporter

Expression

Yeast

Mutation

X=UbiM (see schematic) linker=deltak

Available since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pAAH3

Plasmid

#41827

Purpose

Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.

Depositor

Melissa Moore

Publication

Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Type

Empty backbone

Use

Pcr template

Expression

Yeast

Promoter

TEF

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pZEG

Plasmid

#129112

Purpose

Z. rouxii episomal vector containing G418 resistance

Depositor

Paolo Giudici

Publication

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-A1-FLAG:natMX4

Plasmid

#134432

Purpose

PCR-based C-terminal A1-FLAG tag

Depositor

Robert Tomko

Publication

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

A1-FLAG

Use

Pcr-based tagging in yeast

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pICE ADE2-HphMx

Plasmid

#122895

Purpose

ADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 13, 2019

Availability

Academic Institutions and Nonprofits only

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EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Publication

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-CaURA3

Plasmid

#44886

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-HSV-HIS3MX6

Plasmid

#20774

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-mTID-HA3-KanMX6

Plasmid

#227982

Purpose

To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID

Tags

HA tag

Expression

Yeast

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pPath-Violacein-native

Plasmid

#200001

Purpose

Violacein pathway. Native promoter and CDS sequence

Depositor

Farren Isaacs

Publication

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT GFP CL1

Plasmid

#182512

Purpose

C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CL1 Degron

Tags

GFP

Expression

Yeast

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSP568

Plasmid

#139493

Purpose

Insertion of the pSTL1 promoter and 24 MS2 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Publication

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xMS2sl

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHES553 [prGal1-RAS2(KAN) pnh604]

Plasmid

#103846

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Publication

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pGal1-Ras2(KAN)

Expression

Yeast

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pXNgate22-1HA

Plasmid

#105077

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Publication

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

Plasmid

#72240

Purpose

Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

Depositor

Daniel Zenklusen

Publication

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

GLT1 GAL1p 24xPP7 V3

Expression

Bacterial

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRS303H-Padh-OsTIR1(F74G)

Plasmid

#247625

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Phleomycin

Plasmid

#188930

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Phleomycin

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHO-UAS-mScarlet-I

Plasmid

#183147

Purpose

Yeat two-hybrid fluorecent reporter for genomic integration

Depositor

Assaf Mosquna

Publication

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

mScarlet-I

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L-Leu2 drive

Plasmid

#89578

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

Depositor

George Church

Publication

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Leu2 gene drive

Expression

Yeast

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB3043(gRNA XI-1)

Plasmid

#73285

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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