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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,151 results for: REP

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Showing: 11221 - 11240 of 23151 results

  2. mApple-hCOL1A1

    Plasmid
    #140113
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140113-plasmid-map-sequence-id-296772

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  3. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  7. pαH-S-RRAR-OMICRON

    Plasmid
    #180424
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180424-plasmid-map-sequence-id-352869

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  8. pSCBE2

    Plasmid
    #133965
    Purpose
    Expresses BE2 CRISPR base editor and gRNA scaffold in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Zhong et al bioRxiv 630137
    Insert
    BE2
    Use
    CRISPR and Synthetic Biology
    Available Since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    133965-plasmid-map-sequence-id-266618

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  9. pSABEn

    Plasmid
    #133969
    Purpose
    Expresses ABEn CRISPR base editor and gRNA scaffold in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Zhong et al bioRxiv 630137
    Insert
    ABEn
    Use
    CRISPR and Synthetic Biology
    Available Since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    133969-plasmid-map-sequence-id-266622

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  14. PRS_8-GCaMP8m (AAV2)

    Viral Prep
    #210400-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from PRS_8-GCaMP8m (#210400). In addition to the viral particles, you will also receive purified PRS_8-GCaMP8m plasmid DNA. PRS-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Simon Wiegert
    Article
    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do
    Promoter
    PRSx8
    Available Since
    Feb. 6, 2026
    Availability
    Academic Institutions and Nonprofits only

  18. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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Showing: 11221 - 11240 of 23151 results