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Human PNP his-tagged

Plasmid

#64076

Purpose

Expression of human Purine Nucleoside Phosphorylase

Depositor

Vern Schramm

Article

Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Tags

Histidine tag

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
AAVS1-Puro Xlone-eGFP

Plasmid

#136936

Purpose

donor plasmid for inducible transgene expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

all-in-one tet-on system

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GS

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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SAC1deltaTMD-FKBP-mCherry

Plasmid

#108123

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

SACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oHex

Plasmid

#124670

Purpose

Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHex

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oPent

Plasmid

#124664

Purpose

Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oPent

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB5191

Plasmid

#126911

Purpose

Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

X-4 and XII-5 gRNA

Use

CRISPR

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTagRFP-C1-Fibrillarin

Plasmid

#70649

Purpose

Expresses Fibrillarin nucleolar marker with TurboRFP reporter.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

Fibrillarin (FBL Human)

Tags

TagRFP

Expression

Mammalian

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-gRNA-Actb1216

Plasmid

#155368

Purpose

PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

PspCas13b gRNA targeting Actb 1216

Use

CRISPR

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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EcSTH-LbNOX

Plasmid

#211924

Purpose

This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

EcSTH-LbNOX

Use

Lentiviral

Tags

3xFLAG

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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CMVdel-dimericTomato-WASp(CRIB)

Plasmid

#191450

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET20b-GpNLuc-6xHis

Plasmid

#135932

Purpose

Expresses the GpNLuc LumiFluor in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

GpNLuc LumiFluor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pTNT-B18R

Plasmid

#58978

Purpose

expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pXJ40-Flag-Ferritin-iBox-PAK4cat

Plasmid

#190180

Purpose

expresses Flag-tagged Ferritin-iBox-PAK4cat

Depositor

Bianxiao Cui

Article

Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

Insert

Ferritin-iBox-PAK4cat

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTNT-B18R-6His

Plasmid

#58979

Purpose

expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Tags

His

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93

Plasmid

#87336

Purpose

To express human Nup93 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

Plasmid

#204497

Purpose

Expresses rtTA and Neomycin resistance genes in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Insert

rtTA

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-eLACCO1.1

Plasmid

#167944

Purpose

Fluorescent biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLACCO1.1

Expression

Bacterial

Promoter

araBAD

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pH6HTC_FAM98A-Halo

Plasmid

#175329

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

FAM98A (FAM98A Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-U-TVA950

Plasmid

#115236

Purpose

Lentiviral transfer plasmid for expression of TVA950

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

TVA950

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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