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pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

Plasmid

#114087

Purpose

U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for FnCas12a crRNAs

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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TRP1MX6-ins4

Plasmid

#195041

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

TRP1

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-Myc-PGK-Puro-IRES-GFP

Plasmid

#75124

Purpose

Retroviral expression plasmid encoding wild type murine Myc

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Tags

None

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
mCardinal-MCAK-C-7

Plasmid

#56168

Purpose

Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MCAK (KIF2C Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pHIV-EGFP.Flag-EZH2-mt2*

Plasmid

#220246

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2* (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

PRKKKR494-499NAAIRS

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594N

Plasmid

#131713

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594N

Promoter

CMV

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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CD2 gRNA (BRDN0001146864)

Plasmid

#75827

Purpose

3rd generation lentiviral gRNA plasmid targeting human CD2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CD2 (CD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 4NQ

Plasmid

#121456

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.23 A82V 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86022

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain; Changed alanine 82 to va…

Promoter

CMV IE

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTE4889

Plasmid

#88905

Purpose

Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
hFnCpf1(D917A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgKEAP1-1

Plasmid

#74985

Purpose

sgKEAP1-1, puromycin selection

Depositor

William Hahn

Article

Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

Insert

KEAP1 (KEAP1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

Plasmid

#114089

Purpose

CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized FnCas12a

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-STAT3

Plasmid

#195575

Purpose

Expresses STAT3

Depositor

Kevin Park

Article

Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

Insert

Signal transducer and activator of transcription 3 (Stat3 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CMV enhancer and promoter

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-FKO (NC-flag)

Plasmid

#159363

Purpose

Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS-CoV-2)

Tags

Flag and Flag

Expression

Mammalian

Mutation

Furin-cleavage site mutation

Promoter

CMV

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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CSK gRNA (BRDN0001149071)

Plasmid

#77768

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSK (CSK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-G614 (NC-flag)

Plasmid

#156419

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Mutation

D614G

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-ABCF2_STOP

Plasmid

#221425

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

Insert

ABCF2 (ABCF2 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-rPKM2 WT

Plasmid

#107158

Purpose

MBP-rPKM2 WT for production and purification of rat PKM2 WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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GSK3B gRNA (BRDN0001145264)

Plasmid

#77281

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSK3B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSK3B (GSK3B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GSK3B gRNA (BRDN0001146390)

Plasmid

#77282

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSK3B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSK3B (GSK3B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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