We narrowed to 22,647 results for: eng
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Plasmid#186156PurposesgRNA targeting murine Msh2 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMsh2_gRNA (Msh2 Mouse)
UseCRISPRExpressionMammalianPromoterU6Available sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-evoA1-seBEN_empty
Plasmid#174701PurposeSmall-molecule controlled base editingDepositorInsertLenti-evoA1-seBEN
UseLentiviralTagsmyc tagPromoterEFS/U6Available sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-evoA1-seBEC
Plasmid#174702PurposeSmall-molecule controlled base editingDepositorInsertLenti-evoA1-seBEC
UseLentiviralTagsflag tagMutationUGI-E11KPromoterEFSAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEntr1a-mcherry-FKBP-TIAM1(DHPH)
Plasmid#176135PurposeHeterodimerization with FRB (rapamycin), Rac1 activationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTIAM1 (TIAM1 Human)
UseGateway CloningMutationSee Depositor Comments BelowAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
SspB-mCherry-DHPH-TIAM1
Plasmid#176104PurposeHeterodimerization with iLID, Rac1 activationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTIAM1 (TIAM1 Human)
ExpressionMammalianAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)
Plasmid#168190PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.DepositorInsertHA-nLag6-(EAAAK)4-OGA(544-706)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CMV-Grid2(I677C)
Plasmid#164782Purposecysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligandDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGrid2 I677C (Grid2 Mouse)
ExpressionMammalianMutationI677CPromoterCMVAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD1 tRNA-gRNA position E2 (GB2239)
Plasmid#160561PurposetRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttRNA-gRNA position E2 (Multiplexing Edit)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SBP-GFP-Tac-TC
Plasmid#162506PurposeExpresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
TagsGFP and SBPExpressionMammalianAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
Plasmid#159817PurposePositive control for germline GFP expression from simple array in C. elegansDepositorInsertpEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
ExpressionWormMutationNot applicableAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCIPpay-BFP reporter
Plasmid#154096PurposeSelf-Cutting and Integrating Payload CRISPR backbone with BFP reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP2A-tagBFP
UseCRISPRExpressionMammalianAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg2 (CG510)
Plasmid#139456PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-HCMVgH-8his (Merlin)
Plasmid#136447PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
TagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-716). Codon-optimize…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-GpNLuc
Plasmid#135949PurposeExpresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGpNLuc LumiFluor
TagsT2 mitochondrial localization sequenceExpressionMammalianPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>m2a(silent)-srt-his
Plasmid#124807PurposeHDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMurine IgG2a (Fc silent)
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationL234A/L235A/N297AAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28b Me31B-3Myc
Plasmid#113001PurposeFor protein expression and purification of Myc-tagged full-length Drosophila me31BDepositorAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9
Plasmid#107857PurposeTo knock-out TDP43DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA against TARDBP (Exon 1) (TARDBP Human)
ExpressionMammalianAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3
Plasmid#107731PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
ExpressionMammalianPromoterU6Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
b2-TS5
Plasmid#103878Purposefull-length human b2 with tension sensor at b2_743DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfull-length human b2 with tension sensor at b2_743 (ITGB2 Human)
Tagstension sensor moduleAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-FRB:Akt1(D274A)
Plasmid#86634PurposeExpresses FRB-tagged human Akt1(D274A) kinase-inactive mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFRB-Akt1(D274A) (AKT1 Human)
ExpressionMammalianAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only