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6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

Plasmid

#216387

Purpose

Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Dishevelled-2 (Dvl2 Mouse)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#196545

Purpose

Donor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

Depositor

Lacra Bintu

Article

DelRosso et al Nature. 2023 Apr;616(7956):365-372. doi:

Insert

Surface marker and Citrine reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

Plasmid

#199457

Purpose

Expression of dCas13-VPR in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

dCas13

Use

Lentiviral

Tags

VPR-T2A-HaloTag

Expression

Mammalian

Mutation

Nuclease-dead H133A and H1058A

Promoter

CAG

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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hSyn-somAion-citrine

Plasmid

#192570

Purpose

AAV-vector for optogenetic long-term silencing of neurons

Depositor

Simon Wiegert

Article

Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

Insert

somAion

Use

AAV

Tags

Citrine - soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

human synapsin

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTZ tRNA_gRNA empty scaffold

Plasmid

#188450

Purpose

Expression of gRNAs using a human tRNA promoter

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

tRNA glutamine

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRB112

Plasmid

#180173

Purpose

pAAV construct GluN1WT (shRNA resistant)

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

AAV

Tags

Phluorin

Expression

Mammalian

Mutation

WT

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL3-ABCC8-9-10

Plasmid

#135918

Purpose

Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing variants

Depositor

Diana Valverde Pérez

Article

Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

Insert

ATP-binding Cassette subfamily C member 8 (ABCC8 Human)

Use

Exon trapping

Expression

Mammalian

Promoter

SV40

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCAD54_CAD29_H104A_pvp008

Plasmid

#124876

Purpose

Expresses hCAD His103Ala mutant in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

mutated His103 to Ala, deleted amino acids 1-3 an…

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOP461

Plasmid

#85934

Purpose

Encodes the Anti-Ebola 4G7 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

Depositor

Timothy Lu

Article

Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

Insert

Anti-Ebola 4G7 monoclonal antibody

Expression

Yeast

Promoter

AOX1

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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VPS13C^Halo-△(ATG2C-PH)

Plasmid

#232867

Purpose

Expresses VPS13C-△(ATG2C-PH) in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

VPS13C-△(ATG2C-PH) (VPS13C Human)

Tags

internal Halo

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-VAB(VPS13C)

Plasmid

#232865

Purpose

Expresses the VAB domain of VPS13C in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

the VAB domain of VPS13C (VPS13C Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

Plasmid

#222852

Purpose

Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Tags

IgG1_Fc

Expression

Mammalian

Mutation

L234A, L235A, P329G

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pYDS2.0_AT118-L

Plasmid

#222847

Purpose

Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Expression

Yeast

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBB75-ncRNA

Plasmid

#194087

Purpose

Expresses Ec86 ncRNA in Escherichia coli

Depositor

Tingting Zou

Article

Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

Insert

Ec86 ncRNA

Promoter

T7

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

Plasmid

#176839

Purpose

To recruit SAM complex to human CYP3A4 proximal enhancer.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer sgRNA for activation

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT7_SEPT9_i3-TEV-Strep

Plasmid

#174501

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i3

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)

Tags

TEV-Strep

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A2 D962N

Plasmid

#171821

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP13A2 D962N

Depositor

Veerle Baekelandt

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

human ATP13A2 (ATP13A2 Human)

Use

Lentiviral

Mutation

WT, D508N

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-N

Plasmid

#154424

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

N (N SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available Since

Sept. 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCsy3-VPR-Csy4

Plasmid

#153943

Purpose

Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Insert

Csy3-VPR, Csy4

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCsy1-Csy2

Plasmid

#153942

Purpose

Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Insert

Csy1, Csy2

Use

AAV and CRISPR

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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