We narrowed to 16,079 results for: nes
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Viral Prep#232837-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Syn-NES-SomaFRCaMPi (#232837). In addition to the viral particles, you will also receive purified pAAV-Syn-NES-SomaFRCaMPi plasmid DNA. Synapsin-driven SomaFRCaMPi calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only
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pQP-NiRISB
Plasmid#138977PurposeLight-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)DepositorInsertNcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
Available SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
JPF0419d
Plasmid#124020PurposeEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vectorDepositorInsertPB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pvha6-AgDD
Plasmid#80626Purposecreate protein aggregates in C. elegansDepositorAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX855
Plasmid#62887PurposeN-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)DepositorInsertSpCas9 (aa 2-535)
UseCRISPRTagsFRB and NES PTK2ExpressionMammalianMutationAspartic acide 10 to Alanine (D10A)PromoterCBhAvailable SinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
single polypeptide FPX biosensor for caspase-3
Plasmid#60883PurposeFPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).DepositorInsertsingle polypeptide FPX biosensor for caspase-3
TagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
Plasmid#115684PurposeNanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.DepositorInsertNluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
UseLentiviralAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQP-2460
Plasmid#138973PurposeLight-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1DepositorInsertNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
Available SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
3NES-CS(G)-tTA-CS(G)-3NES
Plasmid#137836PurposeSoluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequencesDepositorInsert3NES-CD(G)-tTA-CS(G)-3NES
ExpressionMammalianPromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)
Plasmid#103849PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.DepositorInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterCMVAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)
Plasmid#103866PurposedPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.DepositorInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…Available SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cyto-ABKAR
Plasmid#61510PurposeExpression of AMPK biosensorDepositorInsertAMPK Biosensor
TagsNESExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-NC
Plasmid#30226DepositorInsertrhVitronectin with N and C terminus cleavages (VTN Human)
ExpressionBacterialMutationdeleted amino acids: 399-478, 20-62Available SinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-N
Plasmid#30225DepositorInsertrhVitronectin with N terminus cleavage (VTN Human)
ExpressionBacterialMutationdeleted amino acids: 20*-62Available SinceMay 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
TagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…Available SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-NmCas9-FLAG
Plasmid#47872PurposeFLAG tagged NmCas9 with no NLS.DepositorInsertNmCas9
UseCRISPRTagsFLAGExpressionMammalianAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
CRISPRi non-coding library (CRiNCL) - Common to all 7 cell lines
Pooled Library#86538DepositorAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN_FL
Plasmid#30223DepositorAvailable SinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRISB
Plasmid#138979PurposeLight-controlled tri-directional targeting of endogenous proteinsDepositorInsertNcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
Available SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only