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(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

Plasmid

#163030

Purpose

Expression of CRISPRi with gSTOP gRNA

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Promoter

Alb-AAT

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD1

Plasmid

#195839

Purpose

Expresses human FZD1 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD1 (FZD1 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCA13-pHR-SFFV-HaloTag-FBL

Plasmid

#199444

Purpose

Expression of HaloTag-FBL in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

FBL (FBL Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pWZ403

Plasmid

#208113

Purpose

mSwAP-In marker cassette 1 with mScarlet-PuroTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-PuroTK

Tags

P2A

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 bovine GRK5

Plasmid

#14690

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

Insert

GRK5 (GRK5 Bovine)

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

Plasmid

#35512

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV2

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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TRBV6-4

Plasmid

#237916

Purpose

Encodes TRBV6-4 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-4-TRAC (TRBV6-4 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV27

Plasmid

#237947

Purpose

Encodes TRBV27 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV27-TRAC (TRBV27 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV19

Plasmid

#237976

Purpose

Encodes TRAV19 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV19 (TRAV19 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV12-2

Plasmid

#237968

Purpose

Encodes TRAV12-2 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV12-2 (TRAV12-2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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Arrb1-RFP

Plasmid

#35401

Depositor

Robert Lefkowitz

Article

Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

Insert

beta-arrestin-1 (Arrb1 Rat)

Tags

RFP

Expression

Mammalian

Promoter

T7

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2 Blast CRLF2

Plasmid

#194090

Purpose

Expression of human CRLF2 gene

Depositor

Martin Carroll

Article

Hurtz et al J Clin Invest. 2020 Jul 1;130(7):3637-36

Insert

CRLF2 (CRLF2 Human)

Use

Lentiviral

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-HA-GOLPH3

Plasmid

#21687

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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mP58.FL1-pCDNA3

Plasmid

#21883

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-S-LexA::p65

Plasmid

#133564

Purpose

Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

LexA::p65

Use

CRISPR

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-C7

Plasmid

#194591

Purpose

Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

C7 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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PB-XL-AncBE4max

Plasmid

#136254

Purpose

Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max

Depositor

Zhaohui Ye

Article

McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

Insert

AncBE4max_GFP

Use

CRISPR; Piggybac dna transposon

Expression

Mammalian

Mutation

The AncBE4max_GFP insert was PCR amplified from a…

Promoter

TRE3G

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_174_neoR

Plasmid

#228935

Purpose

Lentiviral expression of EnAsCas12a

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pv9_TAF3_2xPHD-13XL-BASU

Plasmid

#179414

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3.2xPHD-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

TAF3.2xPHD-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-wild type mKdm2b-Flag

Plasmid

#61739

Purpose

Express wild-type mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Article

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only