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AAV_Cdx2 HMEJ donor

Plasmid

#97319

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Cdx2 HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-6-C, 16-C

Plasmid

#124681

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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p5E-CAGGS (JDW 912)

Plasmid

#224472

Purpose

Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

CAGGS Promoter

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a (JDW 1164)

Plasmid

#224473

Purpose

Gateway compatible 5' entry clone containing the human EF1a promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF1a promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RC(FL)

Plasmid

#46864

Purpose

expresses mouse IL-17RC (all exons)

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RC (Il17rc Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

Plasmid

#97313

Purpose

Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

Lentiviral and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK2

Plasmid

#89363

Purpose

A lenti-viral vector expressing photoswitchable MEK2 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK2 (MAP2K2 Human, Synthetic)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJET-CMV-GFP-P2A-K20-P2A-mCherry

Plasmid

#185618

Purpose

Ribosomal stalling reporter: positive control

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

GFP-K20(AAA)-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-CMV-GFP-P2A-K0-P2A-mCherry

Plasmid

#185619

Purpose

Ribosomal stalling reporter: empty

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

GFP-empty-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-CMV-GFP-P2A-I15-P2A-mCherry

Plasmid

#185620

Purpose

Ribosomal stalling reporter: 15x isoleucine

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

GFP-I15-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ACTN3-pEGFP

Plasmid

#52670

Purpose

Overexpression of GFP-tagged α-actinin-3

Depositor

Johannes Hell

Article

Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

Insert

ACTN3 (ACTN3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)

Plasmid

#214880

Purpose

Lentiviral vector encoding RfxCas13d targeting CD46 guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crCD46-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTargetF-FRT

Plasmid

#113637

Purpose

Derivative of pTargetF with gRNA targeting FRT site

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

sgRNA-FRT

Use

CRISPR

Promoter

pJ23119

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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TRAV26-2

Plasmid

#237984

Purpose

Encodes TRAV26-2 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV26-2 (TRAV26-2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV11-2

Plasmid

#237933

Purpose

Encodes TRBV11-2 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV11-2-TRAC (TRBV11-2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV30

Plasmid

#237987

Purpose

Encodes TRAV30 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV30 (TRAV30 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV4-3

Plasmid

#237907

Purpose

Encodes TRBV4-3 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV4-3-TRAC (TRBV4-3 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-nCas9-UdgX

Plasmid

#163566

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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single polypeptide FPX biosensor for caspase-8

Plasmid

#60884

Purpose

FPX biosensor for caspase-8

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for caspase-8

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_PHC3

Plasmid

#45262

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PHC3 (PHC3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only