We narrowed to 19,528 results for: MUT
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BS.YxiN.Twin1.a1-368.E152Q
Plasmid#17517DepositorInsertB. subtilis DEAD-box helicase YxiN first two domains
Tagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 369-479; E152Q mutat…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)
Plasmid#14851DepositorInsertGAL1 promoter TATA-C2 (GAL1 Budding Yeast)
TagsVYFPExpressionYeastMutationTATA-C2. TATA box mutated from TATATAAG to TCTATA…Available SinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)
Plasmid#14852DepositorInsertGAL1 promoter TATA-C2 (GAL1 Budding Yeast)
TagsECFPExpressionYeastMutationTATA-C2. TATA box mutated from TATATAAG to TCTATA…Available SinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP CCS1(Δ304-316)
Plasmid#139163PurposeSB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1) CCS-CTS deletion mutant; the other side contains RFP and puromycin resistance genes.DepositorInsertcaffeine synthase 1 [Coffea arabica] CCS-CTS region deletion
UseTransposonExpressionMammalianMutationdeleted amino acids 304-319PromoterEF1αAvailabilityAcademic Institutions and Nonprofits only -
His6TEV-UbG76C
Plasmid#75299PurposeBacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV proteaseDepositorInserthuman Ubiquitin mutant G76 to C
TagsHisExpressionBacterialMutationchanged Glycine 76 to CystinePromoterT7Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOlyA(WT)
Plasmid#132391PurposeOstreolysin A for sensing sphingomyelin/cholesterol complexesDepositorInsertOstreolysin A wild-type
TagsHis6 tagExpressionBacterialMutationMutations: C62S, C94S, S151CAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover
Plasmid#196829PurposeExpress dpdpCas13b with AAAA mutation and CloverDepositorInsertdpspCas13b(AAAA)
Tags3XFLAG and NESExpressionMammalianMutationK367A, D369A and K370AAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-N
Plasmid#37533DepositorInsertcysteine-free SGFP2-N
ExpressionMammalianMutationC48S and C70M mutations relative to SGFP2PromoterCMVAvailable SinceAug. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-DRGFP
Plasmid#113193PurposeDR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.DepositorInsertDR-GFP
UseDonor construct with t2a-hygromycin for gene trap…ExpressionMammalianAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDZ2087
Plasmid#92414PurposeExpresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
TagsMBP (self cleaving)ExpressionBacterialMutationL56V/S135G/S219VPromotertacAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM3D(Gq)-mCherry
Plasmid#50460PurposeDouble floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGK415-rtetTAK8N, L131L
Plasmid#165969PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastPromoterpPGK1Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
ExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…Available SinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
Plasmid#20297PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorHas ServiceAAV Retrograde, AAV1, AAV5, AAV8, and AAV9Insertchannelrhodopsin-2
UseAAVTagsmCherryExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable SinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSAM_Bt
Plasmid#112497PurposeTransposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).DepositorInsertsINSeq-adapted mariner transposon
Himar1C9
Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM4D(Gi)-mCherry
Plasmid#50461PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsHA and mCherryMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-ND1-1397C-AD
Plasmid#183892PurposeExpresses TALED_Left-ND1-1397C-AD in mammalian cellsDepositorInsertTALED for ND1 mutation
ExpressionMammalianPromoterpCMVAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-ND1-1397N
Plasmid#183898PurposeExpresses TALED_Right-ND1-1397N in mammalian cellsDepositorInsertTALED for ND1 mutation
ExpressionMammalianPromoterpCMVAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry
Plasmid#50476PurposeGq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only
