We narrowed to 22,846 results for: TES

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pDB2-hCRY2-Halo-CD4-bla

Plasmid

#189986

Purpose

Donor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

HaloTag

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2J1

Plasmid

#124669

Purpose

Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2J1 (UBE2J1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5 TEX264wt-cGFP

Plasmid

#220211

Purpose

Mammalian expression of wild type TEX264 fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Strep-strep-HA

Expression

Mammalian

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta169-189

Plasmid

#187136

Purpose

mammalian cell expression of FLAG-HUWE1 delta169-189

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

delta169-189

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pNIC28-Bsa4_ZV-NS5-RdRp

Plasmid

#179847

Purpose

The RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89

Depositor

Julien Lescar

Article

Gharbi-Ayachi et al J Virol. 2020 Oct 14;94(21). pii: JVI.00

Insert

Zika Virus Non-structural protein 5 RdRp domain

Tags

6-histine

Expression

Bacterial

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc

Plasmid

#208382

Purpose

Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-cMyc T58A

Plasmid

#20075

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

cMyc (MYC Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T58A

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only
pX462-hPRKAA1-gRNA_B

Plasmid

#74375

Purpose

gRNA_B to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla

Plasmid

#174620

Purpose

PiggyBac plasmid expressing EGFP under the control of a cone arrestin promoter

Depositor

Volker Busskamp

Article

Gasparini et al J Clin Invest. 2022 Apr 28. pii: 154619.

Insert

enhanced green fluorescent protein

Use

Piggybac

Expression

Mammalian

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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MoFGFR3b-SV40PA

Plasmid

#86493

Purpose

mamalian cell expression of FGFR3b

Depositor

David Ornitz

Article

Chellaiah et al J Biol Chem. 1994 Apr 15;269(15):11620-7

Insert

FgfrR3 IIIb 12F5 (Fgfr3 Mouse)

Expression

Mammalian

Promoter

Moloney murine leukemia virus LTR

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/empty

Plasmid

#105505

Purpose

Empty backbone of MSCV-driven retroviral cDNA expression

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Type

Empty backbone

Use

Retroviral

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHalo-MyosinIIA-C25

Plasmid

#128573

Purpose

Studies of cellular contractility

Depositor

Dylan Burnette

Article

Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

Insert

Myosin IIA (Myh9 Mouse)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA1-gRNA_A

Plasmid

#74374

Purpose

gRNA_A to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pmiCas9-msa

Plasmid

#164833

Purpose

miCas9-msa fusion protein expressing plasmid

Depositor

Eugene Chen

Article

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Insert

miCas9-msa

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

contains mSA sequence range is 5106-5447 and Brex…

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-FKBP-TACC3-shTACC3

Plasmid

#59354

Purpose

Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

EGFP and FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pTargetF-FRT

Plasmid

#113637

Purpose

Derivative of pTargetF with gRNA targeting FRT site

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

sgRNA-FRT

Use

CRISPR

Promoter

pJ23119

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING (N154S)-EGFP

Plasmid

#214150

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

Insert

STING (STING1 Human)

Use

Retroviral

Tags

EGFP

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-pSFFV-TAF15N-mCherry-sspB

Plasmid

#122436

Purpose

Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

TAF15 (TAF15 Human)

Use

Lentiviral

Tags

mCherry and sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-208

Promoter

SFFV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-CIB1-Myc

Plasmid

#32505

Depositor

John Groffen, Nora Heisterkamp

Article

Haataja et al J Biol Chem. 2002 Mar 8;277(10):8321-8.

Insert

CIB1 (CIB1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
NSP4-WT-Flag

Plasmid

#210339

Purpose

Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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