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pBL4

Plasmid

#50548

Purpose

Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the PcpcG2 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Inserts

CcaS
CcaR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I

Plasmid

#208183

Purpose

mistranslation GFP and mScarlet-I positive control

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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CAG.CenpB(FL)-GFP-Halo

Plasmid

#176205

Purpose

Centromeric anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

CenpB-GFP-Halo

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG 3.1

Plasmid

#193802

Purpose

Protein production of ChemoG3.1 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG 3.1

Tags

10xHis

Expression

Bacterial

Mutation

EGFP: A206K, T225R

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFUM

Plasmid

#133130

Purpose

A recombinant plasmid expressing fum (fumarase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

fumarase gene

Expression

Bacterial

Promoter

PEP-carboxykinase promoter

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pmScarlet3_I3

Plasmid

#239342

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mScarlet3

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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EC67892

Plasmid

#209499

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA6-HA-AML1-ETO

Plasmid

#12508

Depositor

Dong-Er Zhang

Article

Peterson et al Blood. 2007 May 15;109(10):4392-8. Epub

Insert

HA-AML1-ETO (RUNX1T1 Human)

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
SAFB1-pcDNA3.1

Plasmid

#32742

Depositor

Steffi Oesterreich

Article

Townson et al J Biol Chem. 2003 May 30;278(22):20059-6

Insert

SAFB1 (SAFB Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
Sox17 GFP

Plasmid

#23334

Depositor

Sean Morrison

Article

Kim et al Cell. 2007 Aug 10. 130(3):470-83.

Insert

Sex determining region Y-box containing gene 17 (Sox17 Mouse)

Use

Mouse Targeting

Mutation

None

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pPLP1.5

Plasmid

#22614

Depositor

Lynn Hudson

Article

Puckett et al J Neurosci Res. 1987 . 18(4):511-8.

Insert

human Proteolipid Protein cDNA (PLP1 Human)

Use

E. coli phagemid vector

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pEXP1/Joseph2-Multitagged Plasmid

Plasmid

#226724

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in bacterial cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pEXP1/Joseph2

Tags

6xHis, HA, Myc, GFP, Flag, V5

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSBDEST.B

Plasmid

#79460

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Blasticidin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDB007ns2a

Plasmid

#99214

Purpose

Accessory plasmid for PACE of PylRS variants; negative selection

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp, PproK, and Ptet

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-hPGK_NKX2-1

Plasmid

#221494

Purpose

NKX2-1 short isoform lentiviral overexpression using hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

hPGK

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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sfTq2-N1

Plasmid

#117931

Purpose

Superfolder mTurquoise2 (pNM112)

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2

Expression

Mammalian

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-ald*-alaE

Plasmid

#134939

Purpose

Low phosphate inducible alanine dehydrogenase and alanine exporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

alanine dehydrogenase and L-alanine exporter

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pY2-MET-IV

Plasmid

#218117

Purpose

MET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pY2-LYS-IV

Plasmid

#218118

Purpose

LYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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