We narrowed to 15,118 results for: pET

1. pET30a-AlkB-D135S

   Plasmid

   #79051

   Purpose

   Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET259-pUC57 24xMS2V6

   Plasmid

   #104391

   Purpose

   MS2V6 cloning

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-mTurquoise2-CNA35

   Plasmid

   #61602

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mTurqouise and CNA35
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pet-15b-ultraID

   Plasmid

   #172879

   Purpose

   expresses ultraID in E. coli

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   ultraID
   

   Tags

   myc

   Expression

   Bacterial

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15b His-p97

   Plasmid

   #169020

   Purpose

   Expresses His-p97 in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28a mScarlet

   Plasmid

   #178543

   Purpose

   The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

   Depositor

   Siegfried Musser

   Article

   Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

   Insert

   mScarlet
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a SnoopTag-MBP

   Plasmid

   #72323

   Purpose

   Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SnoopTag-MBP
   

   Tags

   N-terminal His6 tag. MBP is fused at the C-termin…

   Expression

   Bacterial

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-6xHis-sfGFP

   Plasmid

   #199171

   Purpose

   used to express a superfolder GFP in E. coli for comparison to supercharged variants

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   superfolder GFP
   

   Tags

   MGHHHHHHGGA

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a T7pCONS sfGFP

    Plasmid

    #154462

    Purpose

    pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Tags

    His-thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-FnCas12a-TEV

    Plasmid

    #174658

    Purpose

    FnCas12a expression plasmid

    Depositor

    Huimin Zhao, Jin Wang

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    FnCas12a
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-SUMO-hGSDMD

    Plasmid

    #111559

    Purpose

    Express human GSDMD in E.coli

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (GSDMD Human)
    

    Tags

    His, SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Sac-Abeta(M1-40)

    Plasmid

    #71876

    Purpose

    Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-40) (APP Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Bxb1-His

    Plasmid

    #123132

    Purpose

    Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Bxb1 integrase
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-Trx-mSA2

    Plasmid

    #52320

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Tags

    FLAG, His, S tag, TEV site, and Thioredoxin

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-LysRS-His

    Plasmid

    #124114

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Lysine-tRNA ligase (lysU Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-Ran(Q69L)

    Plasmid

    #42048

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Ran (RAN Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q69L

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZH82 pET28a-Rai1

    Plasmid

    #231651

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    rai1 (RAI1 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-mH6-Cas12c1

    Plasmid

    #120872

    Purpose

    Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12c1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET-Himar-dCas9

    Plasmid

    #137079

    Purpose

    Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21a-AspRS-His

    Plasmid

    #124106

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Aspartate-tRNA ligase (aspS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21a-ProRS-His

    Plasmid

    #124117

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Prolyl-tRNA ligase (proS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET21a-TyrRS-His

    Plasmid

    #124121

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tyrosyl-tRNA ligase (tyrS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-mH6-Cas12i1

    Plasmid

    #120882

    Purpose

    Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12i1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-6xHis-(-30)GFP

    Plasmid

    #62936

    Purpose

    Expression of 6xHis-(-30)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21-pelB-LaG-16

    Plasmid

    #172746

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET-28a (+)-hTBP

    Plasmid

    #171073

    Purpose

    Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    TATA-box-binding protein (TBP Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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