We narrowed to 2,475 results for: T2A

1. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc

   Plasmid

   #193349

   Purpose

   tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   TetO mini CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124434

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Expression

   Insect

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

   Plasmid

   #132667

   Purpose

   Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-ODD-T2A-TdT (DNTT Streptococcus pyogenes, Human)
   

   Expression

   Mammalian

   Promoter

   4xHRE_YB TATA

   Available Since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

   Plasmid

   #118594

   Purpose

   Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   sgRNA/Puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p226-AAV-dlx-dio-mScarlet-T2A-SYPEGFP

   Plasmid

   #185696

   Purpose

   AAV vector expressing cre-dependent mem-mScarlet, T2A, Synaptophysin-EGFP driven by Dlx promoter

   Depositor

   Lynette Lim

   Insert

   mScarlet, T2A, Synaptophysin-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   mScarlet palmitoylation, Synaptophysin fused to E…

   Expression

   Mammalian

   Promoter

   mDlx

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast

   Plasmid

   #205552

   Purpose

   Express EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP-p6
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4

   Plasmid

   #78349

   Purpose

   Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

   Depositor

   Michael Elowitz

   Article

   Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

   Insert

   H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   rTetR

   Expression

   Mammalian

   Promoter

   pEF alpha

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124431

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Zebrafish (if expression level is too high in zeb…

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73032

   Purpose

   Express fluorescent and luminescence dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   Truncated EF1

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry

    Plasmid

    #83491

    Purpose

    cre-dependent DN-AMPK under EF1a promoter

    Depositor

    Dong Kong

    Article

    Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

    Insert

    DN-AMPK-2A-mCherry
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    K45R

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid

    #109428

    Purpose

    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry T2A GFP
    

    Use

    Lentiviral

    Mutation

    Inserted 43 base pairs into mCherry to create rep…

    Promoter

    CMV

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

    Plasmid

    #174610

    Purpose

    PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    human CD19, antigen minigene
    

    Expression

    Mammalian

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

    Plasmid

    #199197

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int-BlastR
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

    Plasmid

    #175261

    Purpose

    labelling of microtubule plus end and F-actin structures

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
    

    Tags

    mCherry (on iLifeact) and mNeonGreen (on EB3)

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    pTub-alpha1

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199196

    Purpose

    Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR

    Plasmid

    #186740

    Purpose

    Dox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    RAB10, RABIF (RAB10, RABIF Human)
    

    Use

    Lentiviral

    Tags

    RAB10-T2A-RABIF-P2A-mCherry

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170328

    Purpose

    Double-floxed inverse Orientation V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-mir144~451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164793

    Purpose

    Express miR-144~451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144~451 (MIR451A, MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101102

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    TMt-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA and Myc

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

    Plasmid

    #179843

    Purpose

    Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP

    Depositor

    Xiaoping Bao

    Article

    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Luciferase and Synthetic Biology

    Tags

    eGFP and luciferase

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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