We narrowed to 13,683 results for: ckb

1. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

   Plasmid

   #175570

   Purpose

   Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   XbaI site added upstream of U6 promotor. G to C …

   Promoter

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LsgRNA-MS2

   Plasmid

   #235597

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mRuby2-N1

   Plasmid

   #54614

   Purpose

   Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pcDNA3.1-ccdB-3xFLAG-V5

   Plasmid

   #87063

   Purpose

   Mammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

   Depositor

   Mikko Taipale, Susan Lindquist

   Article

   Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

   Type

   Empty backbone

   Use

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSelAct-KO

   Plasmid

   #174374

   Purpose

   Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible

   Depositor

   Gitta Coaker

   Article

   Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

   Type

   Empty backbone

   Use

   Vector for 2-step knockout by homologous recombin…

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mRuby2-Lifeact-7

   Plasmid

   #54674

   Purpose

   Localization: Actin, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Tags

   LifeAct-mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. mOrange2-pBAD

   Plasmid

   #54531

   Purpose

   Localization: Protein Expression Vector , Excitation: 549, Emission: 565

   Depositor

   Michael Davidson, Roger Tsien, Nathan Shaner

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Use

   Tags

   6xHis and mOrange2

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HH-gRNA-HDV-Shuttle-Vector

   Plasmid

   #169098

   Purpose

   Backbone plasmid to generate HH-gRNA-HDV segment

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Development. 2021 Mar 9. pii: dev.193565

   Insert

   HH-gRNAbackbone-HDV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   NA

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pM370

   Plasmid

   #173178

   Purpose

   (Empty Backbone) gRNA-12xMBS

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   gRNA-12xMBS
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mKusabira Orange-C1 (mKO)

    Plasmid

    #54834

    Purpose

    Localization: C1 Cloning Vector, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

    Type

    Empty backbone

    Use

    Tags

    mKusabira Orange

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pMIN-VS1

    Plasmid

    #197783

    Purpose

    Level 2 destination vector: pMIN-VS1 backbone

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mRuby2-pBAD

    Plasmid

    #54771

    Purpose

    Localization: Protein Expression Vector , Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    6xHis and mRuby2

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGWB501NL3F10H

    Plasmid

    #141286

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mRuby2-C1

    Plasmid

    #54768

    Purpose

    Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pHA2

    Plasmid

    #85710

    Purpose

    This plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

    Depositor

    Martin Messerle, Ulrich Koszinowski

    Article

    Adler et al J Virol. 2000 Aug;74(15):6964-74.

    Type

    Empty backbone

    Use

    Cre/Lox; Herpesvirus cloning

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC18T-mini-Tn7T-hph-Ptet1

    Plasmid

    #218367

    Purpose

    Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MiCy-pBAD

    Plasmid

    #54565

    Purpose

    Localization: Protein Expression Vector , Excitation: 472, Emission: 495

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

    Type

    Empty backbone

    Use

    Tags

    6xHis and MiCy

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

    Plasmid

    #137993

    Purpose

    Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC18T-mini-Tn7T-hph-Ptac

    Plasmid

    #218364

    Purpose

    Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS puro-GFP-BamHI-IRES-mCherry

    Plasmid

    #232247

    Purpose

    AcGFP1-BamHI-IRES-mCherry empty backbone

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge