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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,783 results for: cloning

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Showing: 1141 - 1160 of 63783 results

  5. xCas9 3.6

    Plasmid
    #108384
    Purpose
    Mammalian expression vector for xCas9 3.6
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9 3.6
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108384-plasmid-map-sequence-id-207176

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  6. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102245-plasmid-map-sequence-id-354473

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  8. pAC-BETA

    Plasmid
    #53272
    Purpose
    Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Publication
    Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    crtE, crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53272-plasmid-map-sequence-id-487543

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  13. MoClo CRISPR/Cas Toolkit for Plants

    Plasmid kit
    #1000000159
    Purpose
    Plasmids for gene editing using Cas nucleases and base editors in monocots and dicots.
    Depositor
    Vladimir Nekrasov
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Plant Expression
    Editing type
    CRISPR
    Cloning type
    Golden Gate (MoClo)
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only

  14. pmCherry-2xFYVE

    Plasmid
    #140050
    Purpose
    Expresses mCherry-tagged HRS 2xFYVE in mammalian cells
    Depositor
    Harald Stenmark
    Publication
    Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tandem HRS FYVE domain (Hgs Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    two FYVE domains (amino acids 147-223), linked by…
    Promoter
    CMV
    Available since
    May 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140050-plasmid-map-sequence-id-296063

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  17. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107942-plasmid-map-sequence-id-259760

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  18. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid
    #89355
    Purpose
    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs
    Depositor
    Feng Zhang
    Publication
    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cpf1, RR variant
    Use
    CRISPR
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    G532R/K595R
    Promoter
    CMV
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89355-plasmid-map-sequence-id-178226

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  19. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

    Plasmid
    #79620
    Purpose
    Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells
    Depositor
    Akihiko Kondo
    Publication
    Nishida et al Science. 2016 Aug 4. pii: aaf8729.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Tags
    PmCDA1 and UGI
    Expression
    Mammalian
    Mutation
    D10A for nickase Cas9
    Promoter
    pCMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79620-plasmid-map-sequence-id-154120

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Showing: 1141 - 1160 of 63783 results