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pFGL821

Plasmid

#58223

Purpose

For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

hygromycin B phosphotransferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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Cre Stoplight

Plasmid

#45150

Depositor

Thomas Hughes

Article

Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

Insert

Cre Stoplight

Tags

DsRed and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pRsetB-his7-Perceval

Plasmid

#20336

Depositor

Gary Yellen

Article

Berg et al Nat Methods. 2009 Feb;6(2):161-6. doi: 1

Insert

Perceval (fluorescent ATP:ADP sensor)

Tags

his(7)

Expression

Bacterial

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pActP-GCaMP6m

Plasmid

#64714

Purpose

visualizing calcium in cultured Drosophila S2 cells

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

GCaMP6m

Expression

Insect

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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MyrPalm-CFP

Plasmid

#14867

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

CFP

Tags

Myr/Palm

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pFGL822

Plasmid

#58225

Purpose

For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

phosphinothricin acetyl transferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFGL821-8

Plasmid

#58224

Purpose

For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

hygromycin B phosphotransferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pRL1063a

Plasmid

#70687

Purpose

pRL1058 with Vibrio fischeri luxAB for sensing

Depositor

Peter Wolk

Article

Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

Insert

Transposon Tn5-1063

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJ2042.2_invRep

Plasmid

#140688

Purpose

3-color stripe (inverted reporters)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (inverted reporters)

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pROSA26-SAE

Plasmid

#208338

Purpose

ROSA26 targeting vector for the SAE sensor for ERK1/2 signaling

Depositor

Philippe Soriano

Article

Dinsmore et al Dev Biol. 2023 Sep 1:S0012-1606(23)00146

Insert

SAE sensor

Use

Mouse Targeting

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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GLuc VLP

Plasmid

#182213

Purpose

YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).

Depositor

Claudia Vickers

Inserts

VP2C-GLuc
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pPT24-mtnluc

Plasmid

#184339

Purpose

Transformation of mitochondrial DNA in yeast

Depositor

Martin Ott

Article

Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

Insert

nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs

Expression

Yeast

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3

Plasmid

#184901

Purpose

Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-LC3

Plasmid

#184899

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGFP

Plasmid

#32748

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Insert

GFP

Tags

DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL

Expression

Mammalian

Mutation

S65T

Promoter

CMV

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-HaloTag7-mGFP

Plasmid

#184903

Purpose

Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

monomeric EGFP

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pROSA26-1

Plasmid

#21714

Depositor

Philippe Soriano

Article

Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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spCas9-BlastR (pCBhCas9-BlastR)

Plasmid

#71489

Purpose

Expresses SpCas9 and Blasticidin resistance

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

spCas9

Promoter

CBh

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRU1097

Plasmid

#14462

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

gfp-mut3.1

Expression

Bacterial

Available Since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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