We narrowed to 22,641 results for: his
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Plasmid#171780PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-DogTag Loop B
TagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop A
Plasmid#171776PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-SpyTag003 Loop A
TagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues V…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528-AMOT(1-300)-sortase
Plasmid#166706Purposeexpresses AMOT(1-300) with C-terminal sortase tag in bacterial cellsDepositorInsertAMOT aa 1-300
TagsHis-SUMO and Sortase tagExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-deltaH69/V70
Plasmid#171744PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variantDepositorInsertSpike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF3a
Plasmid#169390PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFPDepositorInsertSARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPMutationCodon optimized for Saccharomyces cerevisiaePromoterT7- CMV/CAGAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_Human-ACE2
Plasmid#169406PurposeCell-free/mammalian expression vector for Human ACE2 receptor with N-term eGFPDepositorInsertHuman ACE2 receptor (ACE2 Human)
UseCell-free expression vectorTagsHis and eGFPPromoterT7- CMV/CAGAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL3
Plasmid#169108PurposeExpresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF9b
Plasmid#169396PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF9bwith N-term eGFPDepositorInsertSARS-COV-2 _ORF9b
UseCell-free expression vectorTagsHis and eGFPPromoterT7- CMV/CAGAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_E
Plasmid#169399PurposeCell-free/mammalian expression vector for SARS-COV-2 virus E with N-term eGFPDepositorInsertSARS-COV-2 _E (E SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPPromoterT7- CMV/CAGAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_NSP5
Plasmid#169380PurposeCell-free/mammalian expression vector for SARS-COV-2 virus NSP5 with N-term eGFPDepositorInsertSARS-COV-2 _NSP5
UseCell-free expression vectorTagsHis and eGFPPromoterT7- CMV/CAGAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_NSP2
Plasmid#169377PurposeCell-free/mammalian expression vector for SARS-COV-2 virus NSP2 with N-term eGFPDepositorInsertSARS-COV-2 _NSP2
UseCell-free expression vectorTagsHis and eGFPPromoterT7- CMV/CAGAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS33: pTwinStrep-SUMO-PmcTnsD
Plasmid#168166PurposePmcTnsD expression construct for protein purification.DepositorInsertPmcCAST TnsD
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S S730A
Plasmid#169946PurposePurification of catalytically-dead DPP9S from insect cellsDepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
J-HARS-4
Plasmid#166529PurposescFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
A-WDR5-1
Plasmid#166515PurposescFv of a human scaffold targeting WD repeat domain 5. Antigen coverage aa 1-334 of 334.DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-TTlight-4xDQNAT
Plasmid#128394PurposeIn vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sitesDepositorInsertTetanus toxin light chain domain
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating E234A mutationPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-EPA-DNNNS-DQNRT
Plasmid#128397PurposeIn vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sitesDepositorInsertExotoxin A
Tags6xHis tagExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-TTlight-4xDQNAT
Plasmid#128402PurposePeriplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertTetanus toxin light chain domain
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationInactivating E234A mutationPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-PD-4xDQNAT
Plasmid#128391PurposeIn vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sitesDepositorInsertProtein D
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFE-Rs-FI-prk
Plasmid#162697PurposeExpresses R. spaeroides Form IC rubisco and S. elongatus PrkDepositorInsertR. spaeroides Form IC rubisco and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only