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pGL3-MTS-ND3-Q26-R19-G1397N-UGI-Puro

Plasmid

#198855

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND3-Q26-R19 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND2-W30-R18-G1333N-UGI-Puro

Plasmid

#198853

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND2-W30-R18 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-CYTB-W77-R14-G1333N-UGI-Puro

Plasmid

#198849

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

CYTB-W77-R14 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX3-W16-R15-G1333N-UGI-Puro

Plasmid

#198847

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX3-W16-R15 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro

Plasmid

#198845

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-R18 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX1-W25-R18-G1333N-UGI-Puro

Plasmid

#198843

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX1-W25-R18 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro

Plasmid

#198841

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP8-W9-R15 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ATP6-W68-R17-G1333N-UGI-Puro

Plasmid

#198839

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP6-W68-R17 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND1-W86-L14-G1333C-UGI-Puro

Plasmid

#198850

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND1-W86-L14 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND5-W68-L15-G1333C-UGI-Puro

Plasmid

#198858

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND5-W68-L15 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX3-W16-L17-G1333C-UGI-Puro

Plasmid

#198846

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX3-W16-L17 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro

Plasmid

#198840

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP8-W9-L17 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND3-Q26-L18-G1397C-UGI-Puro

Plasmid

#198854

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND3-Q26-L18 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ATP6-W68-L16-G1333C-UGI-Puro

Plasmid

#198838

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP6-W68-L16 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND4-W24-L18-G1333C-UGI-Puro

Plasmid

#198856

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND4-W24-L18 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

Plasmid

#198844

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-L18 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND2-W30-L16-G1333C-UGI-Puro

Plasmid

#198852

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND2-W30-L16 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro

Plasmid

#198848

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

CYTB-W77-L15 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro

Plasmid

#198842

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX1-W25-L15 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro

Plasmid

#198860

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND6-W82-L15 TALEN

Expression

Mammalian

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-NLS-YFP-CCTc

Plasmid

#184327

Purpose

Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)

Depositor

Xiaodong Liu

Article

Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

Insert

CCTc (CACNA1C Human)

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Pet22b-YAPL318E

Plasmid

#166444

Purpose

Bacterial expression of 6x His tagged YAPL318E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAPL318E (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Pet22b-YAP4LE

Plasmid

#166445

Purpose

Bacterial expression of 6x His tagged YAP4LE

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP4LE (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-neo-YAP-deltaCC

Plasmid

#166469

Purpose

Plasmid coding for YAP-deltaCC (delta298-359) and neomycin resistance in a lentiviral backbone.

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP-deltaCC, Neo (YAP1 Human)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-XZ066-EGFRvIII

Plasmid

#20737

Depositor

Alonzo Ross

Article

Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

Insert

EGFRvIII (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

EGFR with a 267 amino acid deletion in the extrac…

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3/Myc-DNMT1

Plasmid

#36939

Purpose

Mammalian expression of human DNMT1 with myc tag

Depositor

Arthur Riggs

Article

Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

Insert

DNMT1 (DNMT1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDCas9

Plasmid

#71541

Purpose

Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

Depositor

Tao Chen

Article

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Inserts

Cas9
lambda red genes
plasmid curing system

Expression

Bacterial

Promoter

pBAD

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA

Plasmid

#109314

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NSP3-FR-2Strep

Plasmid

#210315

Purpose

Expresses SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3 (ORF1ab SARS-CoV-2)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CAG-dCasRx-RBM25-IRES-BleoR

Plasmid

#221002

Purpose

Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

dCasRx-RBM25-IRES-BleoR

Use

CRISPR

Tags

NLS and NLS-HA

Expression

Mammalian

Promoter

CAG

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT2/CAGXSP/GFPCD63

Plasmid

#231709

Purpose

Stably express CD63 fused with GFP in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Li et al Sci Rep. 2024 Nov 9;14(1):27432. doi: 10

Insert

CD63 (CD63 Human)

Tags

gfp

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV3TAG8li-hRNF160-3FLAG

Plasmid

#159138

Purpose

human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

listerin E3 ubiquitin protein ligase 1 (LTN1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
DDX41-GFP

Plasmid

#175494

Purpose

Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.

Depositor

Evan Braunstein

Article

Li et al Haematologica. 2016 Jun;101(6):e228-31.

Insert

DDX41 (DDX41 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FMR1-E17C-3xFLAG

Plasmid

#157784

Purpose

donor plasmid for C-terminal FLAG tag fusion of human FMR1

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1-FLAG (FMR1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSH-EFIRES-P-AtAFB2-mCherry

Plasmid

#129716

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mPCSK3

Plasmid

#122674

Purpose

Expresses mouse PCSK3 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV3Tag8li-hDDX41-HA

Plasmid

#165469

Purpose

Human DDX41 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DEAD-box helicase 41 (DDX41 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dCpf1-BE

Plasmid

#107685

Purpose

Expresses dLbCpf1-BE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE

Expression

Mammalian

Mutation

D832A/E925A/D1148A

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV3Tag8li-hHP1BP3-HA

Plasmid

#165468

Purpose

Human HP1BP3 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2321 - 2360 of 32708 results

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