We narrowed to 8,248 results for: 11
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Plasmid#85448PurposeExpresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cellsDepositorInsertER50-SpCas9-ER50
UseAAVTagsdestabilized domain of estrogen receptor ligand b…ExpressionMammalianPromoterCbhAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ906
Plasmid#44472DepositorInsertMinimal Mos1, unc-119, [4-3] Gateway
UseGateway CloningAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-p300-DY1399
Plasmid#89095Purposemammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defectDepositorInsertp300-D1399Y mutant (EP300 Human)
TagsHAExpressionMammalianMutationp300-DY1399PromoterSV40Available SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PBS Actb-2A-TIR1-GFP
Plasmid#113832Purposetargeting osTIR1-GFP into mouse Actb locus for auxin inducible degradationDepositorInsertosTIR1-GFP (NEWENTRY Synthetic)
UseSynthetic BiologyAvailable SinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion eGFP
Plasmid#112848PurposeGeneral donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertEGFP
UseUnspecified; General donorPromoterNoAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-hDF
Plasmid#72584Purposehuman dysferlin full length tagged with YFPDepositorAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5
Plasmid#63168PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporterDepositorInsert"spaghetti monster GFP-V5"
TagsN-myristoylationExpressionInsectPromoterGAL4-responsive UAS hsp70Available SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-miRFP703
Plasmid#113097PurposeGeneral donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-miRFP703
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
tubP-FRT>GAL80-6-FRT>
Plasmid#63173Purposeubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassetteDepositorInsert"Flp-Out" GAL80
ExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam N term His8 eGFP 3C
Plasmid#160684PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTagsHis8 eGFP 3CExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR_hNT
Plasmid#71830PurposeExpression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cellsDepositorInsertNon-targeting sgRNA human (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterU6Available SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRES-hrGFP II-TET1_CD
Plasmid#83570PurposeExpresses the catalytic domain of TET1 in mammalian cellsDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
Tags3X FLAG tag and hr GFP IIExpressionMammalianMutationTET1 amino acids 1-1417 deletedPromoterCMVAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP1
Plasmid#129499PurposeExpresses AausFP1 constitutively in E. coli (most strains)DepositorInsertAausFP1
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-synPCB2.1
Plasmid#139478PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
ExpressionMammalianPromoterCAG promoterAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSERT-N1-pEYFP
Plasmid#70105Purposemammalian expression of hSERT with C-terminal YFP fusionDepositorAvailable SinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphC
Plasmid#128137PurposeThe cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphC
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3A9cbetaTCR.20beta
Plasmid#69592PurposeTCR beta chain genomic transgenic construct for creating OT-II miceDepositorInsertTCR beta VJ region from clone 1.1 (Tcrb Mouse)
ExpressionMammalianAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1mTEAD
Plasmid#230916PurposeComparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.DepositorInsertHuman COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
UseLuciferaseTagsLuciferase- luc2pExpressionBacterial and MammalianMutationmutant potential TEAD bind elements AGGAAT to CTG…PromoterCOL1A1Available SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
CYP11A1 (3N9Y)
Plasmid#28119PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCYP11A1:HPC09N-C05:C214491 (CYP11A1 Human)
ExpressionBacterialAvailable SinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQHA-USP11 WT puroR
Plasmid#46749PurposeRetroviral vector that expresses wild type HA-tagged wild type human USP11DepositorAvailable SinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only