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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,350 results for: 11

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Showing: 1161 - 1180 of 8350 results

  1. pES4/4a

    Plasmid
    #69590
    Purpose
    TCR a chain cDNA in pES4 transgenic construct for creating OT-II mice
    Depositor
    Francis R. Carbone
    Publication
    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TCR alpha (Tcra Mouse)
    Expression
    Mammalian
    Promoter
    H-2Kb
    Available since
    Sept. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pEGFPN1-gamma-tubulin

    Plasmid
    #87956
    Purpose
    Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein
    Depositor
    Maria Alvarado-Kristensson, Ana Carrera
    Publication
    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gamma-tubulin 1 (TUBG1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The protein is a sh-gamma-tubulin resistant gene …
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87956-plasmid-map-sequence-id-201686

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  3. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  5. EasyFusion Halo-mAID

    Plasmid
    #112852
    Purpose
    General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein
    Depositor
    Janet Rossant
    Publication
    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Halo-mAID
    Use
    Synthetic Biology
    Promoter
    No
    Available since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112852-plasmid-map-sequence-id-217036

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  6. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid
    #86610
    Purpose
    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86610-plasmid-map-sequence-id-168846

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  8. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid
    #201917
    Purpose
    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Publication
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Expression
    Mammalian
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201917-plasmid-map-sequence-id-403313

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  10. pSFVCs-lacZ

    Plasmid
    #92076
    Purpose
    This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.
    Depositor
    Henrik Garoff
    Publication
    Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C-lacZ
    Use
    C-lacz expression plasmid, for run-off transcript…
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    92076-plasmid-map-sequence-id-181625

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  14. pCAG-HA-rat Nrxn3beta AS4(-)

    Plasmid
    #58269
    Purpose
    Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)
    Depositor
    Peter Scheiffele
    Publication
    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nrxn3beta AS4(-) (Nrxn3 Rat)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58269-plasmid-map-sequence-id-107089

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  17. pKMD111e - intein-Cas9(S219-G521R)

    Plasmid
    #64192
    Purpose
    Expresses intein-Cas9(S219-G521R) in mammalian cells
    Depositor
    David Liu
    Publication
    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
    Use
    CRISPR
    Tags
    NLS - 3x FLAG (C terminal on insert)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    64192-plasmid-map-sequence-id-107551

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Showing: 1161 - 1180 of 8350 results