We narrowed to 5,856 results for: Pet2;

1. pET21a-mAIM2-deltaPYD

   Plasmid

   #51535

   Purpose

   Express His6-tagged HIN domain of mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   Promoter

   lac operator

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28 Set9 mutant

   Plasmid

   #24083

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   H297A

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

3. TrmD_HaeIn/pET28b(+) N,C-tag

   Plasmid

   #12665

   Depositor

   SeWon Suh

   Article

   Kim et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaG-24

   Plasmid

   #172750

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-24
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaG-9

   Plasmid

   #172742

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-9
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET28-FnCas12a-EP16

   Plasmid

   #183636

   Purpose

   PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

   Depositor

   Yunzi Luo

   Article

   Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

   Insert

   FnCas12a-EP16
   

   Use

   CRISPR

   Tags

   6×His Tag

   Expression

   Bacterial

   Mutation

   N607R, K613V, N617R, K180S, K660R, D616N

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-p53 (Mouse 1-87)

   Plasmid

   #62061

   Purpose

   Expression of Mouse p53 (residues 1-87) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

   Insert

   p53 (Trp53 Mouse)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-87

   Promoter

   T7

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET20b- hTPI Lys13Arg

   Plasmid

   #50726

   Purpose

   overexpression of the human TPI1 Lys13Arg allele in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Use

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   changed Lysine 13 to Argenine

   Promoter

   T7

   Available since

   Feb. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-GST-Q11

   Plasmid

   #185790

   Purpose

   Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   GST-Q11
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-8

    Plasmid

    #172761

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-BCA-Pep-H

    Plasmid

    #185782

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Pep-H
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-A774L

    Plasmid

    #174796

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A774L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A774L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28_6xHis-PARP1-W318R

    Plasmid

    #174793

    Purpose

    Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-W318R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    W318, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21a-mAIM2-GST

    Plasmid

    #51533

    Purpose

    Express GST-tagged mAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21b - S. thermophilus DltB

    Plasmid

    #132607

    Purpose

    expresses S. thermophilus DltB in E. coli

    Depositor

    Wenqing Xu

    Article

    Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

    Insert

    Dltb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAR69_STRI_m(KS_MAT)_H8_pET22b

    Plasmid

    #122849

    Purpose

    Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT) (Fasn Mouse)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28_6xHis-PARP1-A762V

    Plasmid

    #174795

    Purpose

    Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A762V (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A762V

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28b-trAtCCD8(pYL736)

    Plasmid

    #178287

    Purpose

    Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    trAtCCD8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a His6Flag2AUF1(p42)

    Plasmid

    #135941

    Purpose

    Expresses AUF1p42 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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