We narrowed to 59,397 results for: cloning

1. C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r

   Plasmid

   #162958

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   triple FLAG epitope tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. C514-E44: attB5r-SNAP-nostop-attB1r

   Plasmid

   #162962

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   SNAP tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

   Plasmid

   #162967

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Dual Strep2 purification tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCoofy62

   Plasmid

   #122014

   Purpose

   Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His6-Smt3Star and Multi3: depending on the LP2 pr…

   Expression

   Mammalian

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. C239-E03: attB2r-IRES-ffLuc2-pA-attB3

   Plasmid

   #162919

   Purpose

   Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   firefly luciferase with upstream IRES sequence
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHBJT011

   Plasmid

   #225162

   Purpose

   Low copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FLAG epitope tag (35 bp)

   Expression

   Bacterial

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBJT008

   Plasmid

   #225159

   Purpose

   Low copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FLAG epitope tag (35 bp)

   Expression

   Bacterial

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHBJT007

   Plasmid

   #225158

   Purpose

   Low copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FLAG epitope tag (35 bp)

   Expression

   Bacterial

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHBJT014

   Plasmid

   #225165

   Purpose

   Low copy cloning vector for integration of C-terminal FLAG epitope tag (SmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FLAG epitope tag (35 bp)

   Expression

   Bacterial

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHBJT013

    Plasmid

    #225164

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (SmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHBJT012

    Plasmid

    #225163

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHBJT010

    Plasmid

    #225161

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (TetR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHBJT009

    Plasmid

    #225160

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (TetR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHBJT006

    Plasmid

    #225157

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHBJT005

    Plasmid

    #225156

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHBJT002

    Plasmid

    #225153

    Purpose

    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r

    Plasmid

    #162936

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Triple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

    Plasmid

    #162937

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6 fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C514-E11: attB5r-RU5u-His6-nostop-attB1r

    Plasmid

    #162953

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6 purification tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r

    Plasmid

    #162956

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Halotag fusion tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge