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Strep-His-Tev-hCGN

Plasmid

#236512

Purpose

For expression of Cingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Human)

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSG5-hEKLF

Plasmid

#67835

Purpose

Expression of human EKLF driven by SV40 promoter for use in activation studies

Depositor

James Bieker

Article

Mas et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

EKLF (KLF1 Human)

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/GFP-ZNF598

Plasmid

#141191

Purpose

Expresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

ZNF598 (ZNF598 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMZ7

Plasmid

#223180

Purpose

pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

Depositor

Paul Blainey

Article

Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

Type

Empty backbone

Use

Chromosomal integration

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSKC12 - DelQ

Plasmid

#110493

Purpose

Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Article

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Insert

NRAS 5'- DelQ (bp 30-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Mutation

Deletion of the first 29 base pairs of the NRAS 5…

Promoter

T7

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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proISG15 (2-165)

Plasmid

#110762

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-RvCAHS3-GS

Plasmid

#192460

Purpose

Expression of RvCAHS3-GS_linker-AcGFP1 in mammalian cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK2

Plasmid

#199641

Purpose

Expresses VRK2

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK2 (VRK2 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pC129: pAAV.CMV-CasRx mCh

Plasmid

#203442

Purpose

Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

RfxCas13d-T2A-mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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POLR2A-N CRISPR pX330

Plasmid

#124495

Purpose

A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

Depositor

Masato Kanemaki

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

POLR2A targeting CRISPR

Use

CRISPR

Promoter

U6 promoter

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGST2-GST-TEV-NDP52

Plasmid

#187828

Purpose

Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Tags

GST-TEV

Expression

Bacterial

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (1-397)

Plasmid

#42205

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (1-397) (SRC Human)

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 1-397

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pFB-EPCP5

Plasmid

#228879

Purpose

Expression of Z-tagged LRRK2 KW using Baculoviruses

Depositor

Sebastian Mathea

Article

Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

His-Z-TEV

Expression

Insect

Mutation

Amino acids deleted 1-1846

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP

Plasmid

#232754

Purpose

Expresses EGFP

Depositor

Isaac Hilton

Article

Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

Insert

EGFP

Use

Lentiviral

Promoter

EF1a

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-TBX20a

Plasmid

#98620

Purpose

Gateway entry TOPO TA cloning vector for TBX20a

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

Tbx20a (Tbx20 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-mJP-4

Plasmid

#79599

Purpose

Transient expression

Depositor

Yousang Gwack

Article

Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

Insert

Junctophilin-4 (Jph4 Mouse)

Tags

2x mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pICE-HA-Ku70-siR-WT

Plasmid

#82329

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

Insert

Ku70 (XRCC6 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV-tet

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-His-TEV-mCherry-OPTN WT

Plasmid

#190191

Purpose

Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

optineurin (OPTN Human)

Tags

6xHis, TEV, and mCherry

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-VPS35

Plasmid

#21689

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-SYN-L-sDarken

Plasmid

#184798

Purpose

Low affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

L-sDarken

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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