168,037 results

1. pAAV-CAG-HcKCR1-EYFP

   Plasmid

   #182021

   Purpose

   Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

   Depositor

   Mingshan Xue

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR1
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT_TO_FlagHA_USP10

   Plasmid

   #127102

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   USP10 (USP10 Human)
   

   Expression

   Mammalian

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MaLionB

   Plasmid

   #113910

   Purpose

   Blue ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   MaLionB
   

   Expression

   Mammalian

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.R_TRE_minCMV

   Plasmid

   #211518

   Purpose

   Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

   Depositor

   Justin English

   Article

   Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   SV40 and minCMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCZGY2254

   Plasmid

   #101247

   Purpose

   GFP subunits 1-10, (only shows active fluorescence when binds to GFP11)

   Depositor

   Yishi Jin

   Article

   Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

   Insert

   gfp1-10
   

   Use

   Gateway

   Tags

   GFP1-10

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV-U6+27-Tornado-RhoBAST

   Plasmid

   #196341

   Purpose

   Overexpression of circular RhoBAST

   Depositor

   Murat Sunbul

   Article

   Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

   Insert

   Tornado-RhoBAST
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. openCIDAR MoClo Toolkit

   Plasmid Kit

   #1000000227

   Purpose

   This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.

   Depositor

   Eric Young

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

8. Dead-Laconic/pcDNA3.1(-)

   Plasmid

   #118627

   Purpose

   To express in mammalian cells a lactate FRET sensor negative control.

   Depositor

   Luis Felipe Barros

   Article

   Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

   Insert

   Dead-Laconic
   

   Expression

   Mammalian

   Mutation

   Change Histidine 151 to Aspartic Acid of Escheric…

   Promoter

   CMV Promoter

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDB364

   Plasmid

   #110275

   Purpose

   N-terminal 10-His DsbC TEV-peptide fusion

   Depositor

   Christopher Bahl

   Article

   Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   10-His, TEV cleavage peptide, and mature DsbC (i.…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1-EGFP-GRAM-H

    Plasmid

    #211703

    Purpose

    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (pLJM1-EGFP-GRAM1b G187L)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mNeonGreen-JIP4

    Plasmid

    #164621

    Purpose

    Expresses JIP4 in mammalian cells with a mNeonGreen tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    JIP4 (SPAG9 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNCS mStrawberry

    Plasmid

    #91768

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Strawberry
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCOLA-Gent-EM7-Erv1p-PDI

    Plasmid

    #202486

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    EM7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 8xNFAT-ZsG-mCD8

    Plasmid

    #162747

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mito-B

    Plasmid

    #209864

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Tags

    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28b-ULP1-405-621

    Plasmid

    #229224

    Purpose

    For expression of ULP1 protein

    Depositor

    Andy LiWang

    Insert

    ULP1 (ULP1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    405-621 (truncated)

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD649-HAsp-HLA-E-COMP5AP-AviTag-9xHis

    Plasmid

    #157396

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    HLA-E (HLA-E Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104907

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTn7-SCOUT11.G

    Plasmid

    #200999

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    superfolder GFP
    

    Expression

    Bacterial

    Promoter

    J23104

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas12j3 VPR

    Plasmid

    #188517

    Purpose

    Expresses FLAG tagged dCas12j3 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D413A

    Promoter

    EF1a

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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