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pHR-EF1Alpha-Tet-on 3G

Plasmid

#118592

Purpose

Expresses Tet-on 3G from the EF1Alpha promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Tet-on 3G

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-A0201-SABR-Backbone

Plasmid

#119050

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Cytosolic Fyn FRET Biosensor

Plasmid

#124654

Purpose

FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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CGBE1 (pRZ3885)

Plasmid

#140252

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pNeuLite

Plasmid

#16247

Depositor

Mien-Chie Hung

Article

Xing et al Nat Med. 2000 Feb . 6(2):189-95.

Insert

Her-2/neu promoter (ERBB2 Human)

Tags

Luciferase

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 S472A

Plasmid

#73052

Purpose

Human AKT3 S472A

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 S472A (AKT3 Human)

Expression

Mammalian

Mutation

S472A

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING

Plasmid

#102598

Purpose

Express STING for IFN-beta luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA HA DUSP6

Plasmid

#236430

Purpose

transient overexpression of DUSP6 in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

Insert

DUSP6 (DUSP6 Human)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe_Hyg_cOVA_T2A_mStrawberry

Plasmid

#161737

Purpose

Expression of cOVA and mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

cytoplasmic ovalbumin

Use

Retroviral

Tags

mStrawberry

Expression

Mammalian

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRClover

Plasmid

#59151

Purpose

Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC24

Plasmid

#231672

Purpose

Expresses human ZDHHC24 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC24 (ZDHHC24 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPSt_35Spr-GFP-t35S

Plasmid

#203592

Purpose

Fully assembled Plant STARR-seq plasmid with the 35S terminator.

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Inserts

CaMV 35S promoter + Zm00001d041672 5'UTR
eGFP
CaMV 35S terminator

Expression

Plant

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn-nucGFP

Plasmid

#140190

Purpose

Overexpression of GFP with a nuclear localization sequence, under the human synapsin promoter

Depositor

Lorenz Studer

Article

Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

Insert

GFP-NLS

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo

Plasmid

#239948

Purpose

Expression of MFSD6 loop 3 tagged with rabbit IgG Fc2

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

Fc2-MFSD6(L3)

Expression

Bacterial and Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5/DEST-VPS35

Plasmid

#21691

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Lentiviral; Invitrogen gateway destination vector

Tags

V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCl-HA-YAP

Plasmid

#27007

Depositor

Joan Massague

Article

Alarcon et al Cell. 2009 Nov 13. 139(4):757-69.

Insert

YAP (YAP1 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only
PEmax_2star_pet21A

Plasmid

#227675

Purpose

Expression plasmid for PEMAX** protein in bacteria.

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

PEmax_2_star

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-SOX4

Plasmid

#110360

Purpose

Mammalian expression of FLAG-tagged SOX4

Depositor

Carlos Moreno

Article

Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

Insert

SOX4 (SOX4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

lacking 5' UTR

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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Opto-CRAC version 1

Plasmid

#101245

Purpose

Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

Depositor

Yubin Zhou

Article

He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

Insert

LOV2 and STIM1 cytosolic fragment

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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