168,037 results

1. pJK02

   Plasmid

   #105133

   Purpose

   Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

   Depositor

   Joseph Sorg

   Article

   McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

   Inserts

   Upstream & Downstream pyrE deletion region
   tetR
   Cas9
   gRNA
   

   Use

   E. coli - c. difficile shuttle vector

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCIDT-attL1-Worm ABeta-attR5

   Plasmid

   #160437

   Purpose

   Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Homo sapiens amyloid beta precursor protein (APP Human, Nematode)
   

   Tags

   Cleavage signal

   Expression

   Bacterial

   Mutation

   Codon-optimized for expression in C. elegans

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

   Plasmid

   #174529

   Purpose

   Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

   Depositor

   Yu-Hsuan Tsai

   Article

   Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

   Inserts

   PylS
   PylT
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-BI

   Plasmid

   #126475

   Purpose

   High-copy number vector for the co-expression of two genes in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-p47PX(aa2-132)

   Plasmid

   #19007

   Depositor

   Michael Yaffe

   Article

   Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

   Insert

   p47phox (NCF1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa2-132 (PX domains)

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pGL410_INS421

   Plasmid

   #49057

   Purpose

   Expresses luciferase under control of human insulin gene promoter

   Depositor

   Kevin Ferreri

   Article

   Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

   Insert

   human INS promoter (INS Human)
   

   Expression

   Mammalian

   Promoter

   INS promoter

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP943

   Plasmid

   #104157

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fab specific nanobody TP886
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PNCA-GFP

   Plasmid

   #67789

   Purpose

   Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

   Insert

   MMLV-LTR-GFP
   

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pHCMV-SS-antiFLAGscFv-TM

   Plasmid

   #198303

   Purpose

   Displays anti-FLAG scFv from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiFLAGscFv-TM
   

   Expression

   Mammalian

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-mDlx-ChR2-mCherry-Fishell-3

    Plasmid

    #83898

    Purpose

    ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    ChR2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-EGFR-E746_T751delinsA

    Plasmid

    #116246

    Purpose

    Lentiviral expression of EGFR E746_T751delinsA

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Mutation

    E746_T751delinsA

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nuclear Myosin I YFP

    Plasmid

    #60241

    Purpose

    Expresses nuclear isoform of myosin IC attached to YFP and an NLS

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-mutFAKSPARK2

    Plasmid

    #195264

    Purpose

    express Tyr to Phe mutant FAKSPARK2

    Depositor

    Xiaokun Shu

    Article

    Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

    Insert

    Tyr to Phe mutant FAKSPARK2 sensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid

    #71657

    Purpose

    mammalian expression of eiF4A1

    Depositor

    Gideon Dreyfuss

    Insert

    eiF4A1 (EIF4A1 Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    aa403 from Ala to Arg

    Promoter

    CMV

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF-5xT7-TtCsm

    Plasmid

    #128572

    Purpose

    For expression of T. thermophilus Csm in E. coli (Csm1-5)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Csm1
    Csm2, Csm3, Csm4, Csm5
    

    Tags

    HRV 3C protease cleavage site and 10xHis tag on C…

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli and Cod…

    Promoter

    T7 promoter, lac operator

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG424-10X-tRNA

    Plasmid

    #70125

    Purpose

    Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

    Depositor

    Jay Hesselberth

    Article

    Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

    Insert

    10X-tRNA block
    

    Expression

    Yeast

    Promoter

    None – SUP4 pol III promoter provided by insert

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

17. SPHK1 gRNA (BRDN0001147534)

    Plasmid

    #76814

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-NPC1L1_STOP

    Plasmid

    #161469

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    NPC1L1 (NPC1L1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. SPHK2 gRNA (BRDN0001146882)

    Plasmid

    #77096

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-U6-gRNA-EF1a-EGFP-CAAX

    Plasmid

    #167936

    Purpose

    gRNA entry vector

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    U6-gRNA backbone
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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