We narrowed to 32,294 results for: uros

1. pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

   Plasmid

   #183605

   Purpose

   Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   EGFP-TM
   

   Tags

   HA, Human PDGFRB transmembrane domain, Mouse IgGK…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPRv2-dADAM17 (#1)

   Plasmid

   #177259

   Purpose

   A knockout vector for the dog Adam17

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF NS34a-msGFP-'S-mito

   Plasmid

   #158765

   Purpose

   Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Expression

   Mammalian

   Mutation

   Flexible linkers between the protease and msGFP a…

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJR025

   Plasmid

   #198832

   Purpose

   Actin filament and cell cortex localization.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::act2-venus::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDECKO_TFRC_B

   Plasmid

   #72626

   Purpose

   Expresses two gRNAs targeting the TFRC promoter

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward TFRC
   

   Expression

   Mammalian

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. GammaPCDH-A3-GFP/pEGFP-N1

   Plasmid

   #197343

   Purpose

   Plasmid expressing an antigen targeting Pan-Gamma-protocadherin-Constant

   Depositor

   Joshua Weiner

   Article

   Lobas et al J Neurochem. 2012 Mar;120(6):913-27. doi

   Insert

   pcdh-gamma-A3
   

   Tags

   GFP (GFP observed)

   Expression

   Mammalian

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgRNA3_GAL4UAS-Luciferase reporter

   Plasmid

   #64159

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICE-EGFP-FLAG-Ku70siR-WT

   Plasmid

   #46961

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   human Ku70 (XRCC6 Human)
   

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV Tet

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF(UG) hSyn PP-HaloTag-SYT7alpha

   Plasmid

   #179714

   Purpose

   Lentiviral expression of mouse Syt7 with N terminal HaloTag

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pF(UG) hSyn PM-SYT7alpha deltaTMD

    Plasmid

    #179720

    Purpose

    Lentiviral expression of plasma membrane-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SEPTOPO

    Plasmid

    #64944

    Purpose

    source of SEP (Super Ecliptic pHluorin) sequence

    Depositor

    Jeremy Henley

    Article

    Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

    Insert

    SEP
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pTYF-super-mfvAChT-1.8k-Venus-WPRE

    Plasmid

    #156389

    Purpose

    Expresses Venus under vAChT gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWB524

    Plasmid

    #74866

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLib6.4B-Agam_695

    Plasmid

    #176668

    Purpose

    Expression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWB529

    Plasmid

    #74871

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. p045

    Plasmid

    #70057

    Purpose

    Co-expresses Syt1-SNARE37aa-linker complex with pDC184

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

    Inserts

    C2AB-linker-SNAP-25_C
    SNAP-25_N
    

    Tags

    No tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB443

    Plasmid

    #74829

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. RT3GEP

    Plasmid

    #111164

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Mutation

    WT

    Promoter

    T3G

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDECKO_Malat1_B

    Plasmid

    #72621

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Expression

    Mammalian

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-lxlplap

    Plasmid

    #45581

    Depositor

    Nirao Shah

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Inserts

    Green fluorescent protein
    Placental alkaline phosphatase (ALPP Human)
    

    Use

    Lentiviral

    Tags

    Histone 2B

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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