We narrowed to 50,550 results for: kan
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Plasmid#73407PurposeExpresses His tagged LanCL2DepositorAvailable sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pMD19T-slr0230-PL22-sgRNANT1-KmR
Plasmid#73224PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorPublicationInsertPL22-sgRNA NT1
ExpressionBacterialPromoterPL22Available sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBbE1k-3
Plasmid#71722Purposetyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humanDepositorPromoterpTrcAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DNMT1
Plasmid#25165PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceApril 4, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSpp1-is3
Plasmid#58247PurposeExpression vector producing isoform 3 derived mouse osteopontin (EGFP tagged)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpp1 isoform 3 (Spp1 Mouse)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME eos
Plasmid#73792PurposePhotoconvertible eos in a middle entry cloneDepositorInsertEOS
UseMiddle entry cloneAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGB 35S:dCas9:Tnos (GB1191)
Plasmid#68223PurposeTranscriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoterDepositorInsertdCas9
UseCRISPR and Synthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removed; human codon optimis…Promoter35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
G-GECO1.2-Orai1Y80E
Plasmid#73565PurposeGreen fluorescent reporter of mutant Orai1Y80E-associated calcium influxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG-GECO1.2-Orai1Y80E (ORAI1 Human, Synthetic)
TagsG-GECO1.2ExpressionMammalianMutationchanged tyrosine 80 of Orai1 to glutamatePromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TOPO pTRE
Plasmid#68457PurposeTOPO construct expressing pTRE for generation of GMAP-compatible promoterDepositorInsertTetracycline Response Element Promoter
UseGmapExpressionBacterialPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO rtTA3-2A-Cre
Plasmid#68448PurposeTOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible geneDepositorInsertrtTA3-2A-Cre
UseGmapExpressionBacterialMutationCodon optimized for expression in micePromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPTN425-577-EGFP
Plasmid#68843Purposedeleted OPTN was inserted into pEGFP-N1 backboneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertoptineurin (OPTN Human)
TagsEGFPExpressionMammalianMutationaa 425-577 onlyPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
LEXY TR (with KRAB domain) (pDN163)
Plasmid#72662PurposeLexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY construct for light control of transcriptional repressionDepositorInsertLexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY
Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pME-eSIBR
Plasmid#71665Purposeintronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)DepositorInsertintronic eSIBR cassette
UseRNAi; Multi-site gatewayPromoterNoneAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2NS-IRES-dOrai-IRES-GFP
Plasmid#72895PurposeMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdmBACCS2NS-IRES-dOrai-IRES-GFP
TagsIRES-GFPExpressionMammalianPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dmBACCS2VL-IRES-dOrai-IRES-mCherry
Plasmid#72898PurposeMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdmBACCS2VL-IRES-dOrai-IRES-mCherry
TagsIRES-mCherryExpressionMammalianPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Kif5cmut
Plasmid#71854Purposemammalian expression of mutant GFP-Kif5cDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKif5c (Kif5c Rat)
TagsEGFPExpressionMammalianMutationT93N mutation and also a novel SpeI site (1652). …PromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 VAMP2 R125TAG
Plasmid#69876Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2-GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVAMP2-GFP (Vamp2 Rat)
UseSynthetic BiologyTagsGFPExpressionMammalianMutationAmber stop codon in the linker between VAMP2 and …Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDCAF3-IS5
Plasmid#65220PurposeExpresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.DepositorInsertsndmA
ndmB
ndmC
ndmD
gst9
chlR
UseSynthetic BiologyExpressionBacterialMutationChanged start codon from gtg to atg and Changed s…PromoterBBa_J23100Available sinceJan. 8, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pET28a-HsSENP3(307-574)
Plasmid#71481PurposeEncodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 3 (SENP3 Human)
TagsHexahistidineExpressionBacterialMutationDeleted amino acids 1-306 (catalytic domain only)PromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-S305A-3xFLAG
Plasmid#69839PurposeThis plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPLK4 (PLK4 Human)
Tags3xFLAG tag and EGFPExpressionMammalianMutationS305APromoterCMVAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only