168,037 results

1. pD649-HAsp-TNFSF13B-COMP5AP-AviTag-9xHis

   Plasmid

   #157213

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   TNFSF13B (TNFSF13B Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. RCAS.Sfi

   Plasmid

   #196377

   Purpose

   Empty avian expression vector RCAS with modifications for fixed orientation cloning into SfiI sites

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Type

   Empty backbone

   Use

   Retroviral; Avian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH3717

   Plasmid

   #191477

   Purpose

   Pacr-2s-Arch::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFP

   Depositor

   Mei Zhen

   Article

   Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

   Insert

   Arch (arch-1 Nematode)
   

   Tags

   wCherry

   Expression

   Worm

   Promoter

   Pacr-2s

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPHK1 gRNA (BRDN0001148403)

   Plasmid

   #76815

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK1 (SPHK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLY100-RetroEFS-HER2-28BBz-WPRE

   Plasmid

   #192201

   Purpose

   Retro-HER2CAR

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Retro-HER2CAR (ERBB2 Synthetic)
   

   Use

   Retroviral; Mammalian expression

   Mutation

   NA

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSPL

   Plasmid

   #110743

   Purpose

   Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

   Depositor

   Tim Richmond

   Article

   Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   Polyhedrin and p10 promoters

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINF

   Plasmid

   #26042

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   His3C-lacZ
   

   Tags

   MAHHHHHHSSGLEVL

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-TRPV1exCellHalo

   Plasmid

   #220307

   Purpose

   Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

   Depositor

   Eric Senning

   Article

   Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

   Insert

   rTRPV1exCellHalo (Trpv1 Rat)
   

   Tags

   circularly permutated Halotag (Deo et al., Nat Ch…

   Expression

   Mammalian

   Mutation

   linker included to insert cpHaloTag (

   Promoter

   CMV promoter

   Available Since

   July 30, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. p4xSTAT6-Luc2P

   Plasmid

   #35554

   Depositor

   Axel Nohturfft

   Insert

   4xStat6 promoter
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   4xStat6

   Available Since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWM_12x601_45bpLinker

    Plasmid

    #157791

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_45bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p206-Switch-ON

    Plasmid

    #217885

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-dLight1.3b

    Plasmid

    #111056

    Purpose

    Expresses dLight1.3b in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.3b
    

    Tags

    Flag tag

    Expression

    Mammalian

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBCW

    Plasmid

    #62658

    Purpose

    Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    PhiC31
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK2-AraE

    Plasmid

    #110141

    Purpose

    Constitutive promoter expressing araE on RK2 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    araE
    

    Expression

    Bacterial

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-FOXC1

    Plasmid

    #153114

    Purpose

    Expression of Flag tagged human FOXC1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXC1 (FOXC1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCDM

    Plasmid

    #110742

    Purpose

    Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    Polyhedrin and p10 promoters

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-smURFP

    Plasmid

    #80349

    Purpose

    Lentiviral transfer vector under the CMV promoter that expresses smURFP.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Insert

    smURFP
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pERM2-nhpSer

    Plasmid

    #201922

    Purpose

    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    E412P, E414F, T417K, P495M, I496W, F529S and H67R…

    Promoter

    GlnS (constitutive), OXB20 (constitutive), lpp (c…

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGAselect

    Plasmid

    #195714

    Purpose

    pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGL-FLKif3A

    Plasmid

    #13742

    Depositor

    Linda Wordeman

    Article

    Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

    Insert

    mouse Kif3A (Kif3a Mouse)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Jan. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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