178,515 results
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Plasmid#177142PurposeBacterial vector for expression of an N-terminal GST fusion of the Tev proteaseDepositorInsertTev
TagsGSTExpressionBacterialPromoterTacAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VPS13D^EGFP
Plasmid#174109PurposeThe insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.DepositorInsertCodon optimized VPS13D
TagsEGFPExpressionMammalianMutationWith an internal EGFP tag in aminoacid position 1…PromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)
Plasmid#175573PurposeInactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repressionDepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mouse Cherry Brie Pooled Library
Pooled library#170511PurposeGenome-wide knockout library. It is identical to the Brie library #73633, replacing Puromycin resistance with mCherry.DepositorPublicationExpressionMammalianSpeciesSyntheticUseCRISPR and LentiviralAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX-GST-His6-2_HBD
Plasmid#171870PurposeExpresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cellsDepositorInsertTandem repeats Hybrid binding domain of Rnase H1
TagsGST- and His10-tagExpressionBacterialMutationWTAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU295
Plasmid#167694PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONRp5-p2 SspB-mCherry-CShroom3
Plasmid#170977PurposeC-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.DepositorInsertCShroom3 (Shroom3 Mouse)
UseGateway CloningTagsSspB and mCherryMutationIsoform X2, deleted aminoacids 1 - 1388Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2
Plasmid#166014Purposemammalian expression of HCoV-HKU1 soluble spike trimer proteinDepositorInsertHCoV-HKU1 S (spike)
Tags3C-His8-Strep2x2ExpressionMammalianPromoterCMV51Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_NEmut (AAV9)
Viral prep#123310-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NEmut (#123310). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NEmut plasmid DNA. hSyn-driven expression of GRAB-NEmut control norepinephrine sensor (does not respond to NE). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 D614G
Plasmid#164437PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus productionDepositorInsertSARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )
ExpressionMammalianAvailable sinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AiP11633 - pAAV-hsA2-AiE0140h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1633)
Plasmid#163503PurposeDirect-expressing SYFP2 AAV VirusDepositorInsertSYFP2
UseAAVPromoterMinRhoAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nsp4-mCherry
Plasmid#165132Purposemammalian expression and localizationDepositorAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nsp6-mCherry
Plasmid#165133Purposemammalian expression and localizationDepositorAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2
Plasmid#164514PurposeGreen glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244084) instead of this plasmid.DepositorInsert(Cyto)iGlucoSnFR-mRuby2
UseAAVMutationSynthetic ConstructPromoterGFAPAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OMMGFP 1-10
Plasmid#164119PurposeExpresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMMDepositorInsertOMMGFP 1-10
ExpressionMammalianAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-mCherry-Nanobody (LaM-2)
Plasmid#162276PurposeExpresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.DepositorInsertmCherry-Nanobody (LaM-2)
TagsGSTExpressionBacterialAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn Con/Fon EYFP (AAV Retrograde)
Viral prep#55650-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon EYFP (#55650). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon EYFP plasmid DNA. Synapsin driven, Cre and Flp-dependent EYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXP3
Plasmid#153147PurposeExpression of Flag tagged human FOXP3 in mammalian cellsDepositorAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRDA_078
Plasmid#158582PurposeLentiviral expression of BE4max and customizable gRNADepositorTypeEmpty backboneUseLentiviralExpressionMammalianMutationCas9 D10AAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETduet+p300HAT
Plasmid#157793PurposeExpression of p300 HAT domainDepositorAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by