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Addgene

We narrowed to 7,257 results for: Trac;

Showing: 101 - 120 of 7257 results
  1. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid
    #138525
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.
    Depositor
    Insert
    Control non-PRC2 binding A-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid
    #138524
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.
    Depositor
    Insert
    PRC2-binding G-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Mouse, Human, Bovine)
    TRAC-3' homology arm (TRAC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid
    #49069
    Purpose
    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C563S,C568S,C577S,C582S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

    Plasmid
    #49080
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C791S, C820S, C1052S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Nov. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
  6. NCOR2

    Plasmid
    #40737
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    NCOR2 (NCOR2 Human)
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Residues 615 to 685 of the SANT2 domain of Nuclea…
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. TRBV27

    Plasmid
    #237947
    Purpose
    Encodes TRBV27 allele and TRAC to generate TCRs via cloning
    Depositor
    Insert
    TRBV27-TRAC (TRBV27 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. TRBV28

    Plasmid
    #237948
    Purpose
    Encodes TRBV28 allele and TRAC to generate TCRs via cloning
    Depositor
    Insert
    TRBV28-TRAC (TRBV28 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 120 of 7257 results