We narrowed to 25,243 results for: cDNA

1. pCDNA3_Gap43_Csx30_Cre

   Plasmid

   #197604

   Purpose

   Cre reporter for mammalian cells with Gap43 membrane anchor

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   Gap43_Csx30_Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3_Chrm3_Csx30_Cre

   Plasmid

   #197603

   Purpose

   Cre reporter for mammalian cells with Chrm3 membrane anchor

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   Chrm3_Csx30_Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcdna3mciap1

   Plasmid

   #11474

   Depositor

   Jon Ashwell

   Article

   Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

   Insert

   mciap1 (Birc2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 8, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3_CDC50A

   Plasmid

   #203694

   Purpose

   expresses CDC50A

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   TMEM30A (TMEM30A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ciCas9_pcDNA5

   Plasmid

   #100550

   Purpose

   Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   ciCas9
   

   Use

   CRISPR; Frt, flp-in

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_ERBB2-V5

   Plasmid

   #214612

   Purpose

   ERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment assay

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mOtop2_pcDNA3

   Plasmid

   #114678

   Purpose

   This construct was used to express mouse Otop2 in HEK-293 cells

   Depositor

   Emily Liman

   Article

   Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

   Insert

   Otopetrin 2 (Otop2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1.H1-NP

   Plasmid

   #134367

   Purpose

   Nanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   H1-NP (HRH1 Human)
   

   Use

   Tags

   Flag and natural peptide of nanoluciferase

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA_DiCre2_60.F2

   Plasmid

   #60210

   Purpose

   Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)

   Depositor

   Jean-Paul Herman

   Insert

   Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)
   

   Use

   Tags

   FKBP12(F36V) and Nuclear localisation signal

   Expression

   Mammalian

   Mutation

   FKBP12 has the F36V mutation; amino acids 60-343 …

   Promoter

   CMV

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3_HA_Csx30

    Plasmid

    #197610

    Purpose

    Expresses a HA-tagged version of Csx30

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    HA-tagged Csx30
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_27F3_hFc

    Plasmid

    #199619

    Purpose

    Encodes DARPin-hFc 27F3 for mammalian expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-hFc 27F3
    

    Use

    Tags

    hFc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_hMed25

    Plasmid

    #206958

    Purpose

    The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

    Depositor

    Reza Zolfaghari, Catharine Ross

    Insert

    Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_hE2F7mg

    Plasmid

    #73073

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1dsRluc

    Plasmid

    #68053

    Purpose

    expresses destabilized Renilla Luciferase

    Depositor

    Christopher Sullivan

    Article

    Seo et al J Virol. 2008 Oct;82(20):9823-8. doi: 10

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. HfACR1_EYFP_pcDNA3.1

    Plasmid

    #161026

    Purpose

    Expresses strongly red-shifted HfACR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

    Insert

    HfACR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mOtop3_pcDNA3

    Plasmid

    #114679

    Purpose

    This construct was used to express mouse Otop3 in HEK-293 cells

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 3 (Otop3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3.1_CoumRS

    Plasmid

    #64882

    Purpose

    Expresses Coumarinyl- tRNA synthetase

    Depositor

    Sebastián Brauchi

    Article

    Steinberg et al bioRxiv, 10.1101/065227

    Insert

    Coum_RS
    

    Use

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA_NEPTUNE_hTLN2-FL

    Plasmid

    #164842

    Purpose

    Expression of NEPTUNE tagged FL human talin2

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN2 (TLN2 Human)
    

    Use

    Tags

    His10-NEPTUNE

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. A1ACR1_EYFP_pcDNA3.1

    Plasmid

    #161025

    Purpose

    Expresses strongly red-shifted A1ACR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

    Insert

    A1ACR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-ET

    Plasmid

    #72542

    Purpose

    expresses FLAG tagged human BRD4 ET domain (608-699 aa)

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    BRD4 ET (BRD4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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