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We narrowed to 1,731 results for: plasmids pcas

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Showing: 101 - 120 of 1731 results

  1. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  2. pCAG-ORF3cStrep

    Plasmid
    #208728
    Purpose
    Expresses ORF3c-Strep in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    Strep
    Expression
    Mammalian
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    208728-plasmid-map-sequence-id-420909

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  3. pCAG-ORF3cHA

    Plasmid
    #208653
    Purpose
    Express ORF3c-HA in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    208653-plasmid-map-sequence-id-420908

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  4. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Article
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has Service
    Cloning Grade DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71814-plasmid-map-sequence-id-177023

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  5. pCAG-EnvARGCD

    Plasmid
    #100800
    Purpose
    express fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domain
    Depositor
    Ian Wickersham
    Insert
    fusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Dec. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    100800-plasmid-map-sequence-id-189516

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  6. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

    Plasmid
    #127908
    Purpose
    WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene
    Depositor
    Mark Groudine
    Insert
    gRNA for Human 5' HP1beta
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127908-plasmid-map-sequence-id-249780

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  7. pAAV-MP-84-spCas9-U6-gRNA

    Plasmid
    #206935
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206935-plasmid-map-sequence-id-407781

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  8. cacnb1b pCAGGS

    Plasmid
    #206102
    Purpose
    expression of rat beta1b calcium channel auxillary subunit
    Depositor
    Annette Dolphin
    Insert
    cacnb1b (Cacnb1 Rat)
    Expression
    Mammalian
    Promoter
    CMV/B-actin
    Available Since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    206102-plasmid-map-sequence-id-413014

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  9. cacna2d1 pCAGGS

    Plasmid
    #206104
    Purpose
    expression of rat alpha2delta-1 calcium channel auxillary subunit
    Depositor
    Annette Dolphin
    Insert
    cacna2d1 (Cacna2d1 Rat)
    Expression
    Mammalian
    Promoter
    CMV/B-actin
    Available Since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    206104-plasmid-map-sequence-id-413011

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  10. pCAGGS-S-P1-FLAG

    Plasmid
    #177095
    Purpose
    P1 variant S-protein with c-terminal FLAG tag
    Depositor
    Daniel Conway
    Insert
    S-protein P1 (S SARS-CoV-2)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177095-plasmid-map-sequence-id-345528

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  11. pCAG-GFP-CD63

    Plasmid
    #194989
    Purpose
    overexpress CD63
    Depositor
    Angelique Bordey
    Insert
    GFP-CD63
    Expression
    Bacterial
    Available Since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194989-plasmid-map-sequence-id-387085

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  12. pCAG-GFP-Rab27a

    Plasmid
    #194985
    Purpose
    overexpress Rab27a
    Depositor
    Angelique Bordey
    Insert
    GFP-Rab27a
    Expression
    Bacterial
    Available Since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194985-plasmid-map-sequence-id-386802

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  13. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Quanjiang Ji
    Article
    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    98211-plasmid-map-sequence-id-485072

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  14. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214120-plasmid-map-sequence-id-423617

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  15. pCAG-EGFP-Snapin

    Plasmid
    #118743
    Purpose
    Expresses EGFP tagged Snapin
    Depositor
    Beverly Koller
    Insert
    Snapin cDNA (Snapin Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118743-plasmid-map-sequence-id-229091

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  16. pPB-spCas9-pZeo

    Plasmid
    #214121
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214121-plasmid-map-sequence-id-423619

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  17. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  20. Gli3ZF in AINGpCAGGS

    Plasmid
    #121972
    Purpose
    Gli3 zinc fingers
    Depositor
    James Briscoe
    Insert
    Gli3ZF (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus and C terminus deletion only nt 1411-1…
    Available Since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121972-plasmid-map-sequence-id-241924

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Showing: 101 - 120 of 1731 results