We narrowed to 63,277 results for: tro

1. Cdk12_intron3_sg2_pX330

   Plasmid

   #127174

   Purpose

   sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cdk12_intron4_sg1_pX458

   Plasmid

   #127175

   Purpose

   sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 4 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-Control-miR-17-5p-Control

   Plasmid

   #21167

   Depositor

   Joshua Mendell

   Article

   O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

   Insert

   miR 17-5p Sensor (MIR17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pGL3-Control-miR-20-Control

   Plasmid

   #21169

   Depositor

   Joshua Mendell

   Article

   O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

   Insert

   miR-20 Sensor (MIR20A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Sensor construct insert has two miR-20 binding si…

   Promoter

   Available since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

   Plasmid

   #122085

   Purpose

   Express M.EcoGII fused to CENPC with destabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of CENPC

   Expression

   Bacterial and Mammalian

   Mutation

   L341F, R930L (see comments below)

   Promoter

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVITRO1-dV-IgE/κ

   Plasmid

   #61878

   Purpose

   Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin Epsilon constant region
   Human immunoglobulin kappa constant region
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   mEF1 Prom and rEF1 Prom

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-Citron1

   Plasmid

   #134303

   Purpose

   Mammalian expression of direct-response citrate sensor

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citron1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pVITRO1-dV-IgE/λ

   Plasmid

   #61879

   Purpose

   Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin Epsilon constant region
   Human immunoglobulin lambda constant region
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   mEF1 Prom and rEF1 Prom

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6.3>Control.gRNA.f+e

   Plasmid

   #99140

   Purpose

   Control gRNA under the regulation of an optimized chicken specific U6 promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   Control gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Chicken U6.3

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR v2-sgControl

    Plasmid

    #125836

    Purpose

    KO control

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    Control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNAintron-EBOV-GP

    Plasmid

    #215692

    Purpose

    Produces the Ebola virus glycoprotein

    Depositor

    Melinda Brindley

    Article

    Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

    Insert

    EBOV-GP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. LCV2 control

    Plasmid

    #217443

    Purpose

    Lentiviral vector expressing Cas9 without a targeting sgRNA

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p-AAV-sh[control]

    Plasmid

    #75438

    Purpose

    control shRNA vector

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    GFP
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5_EGFP-control

    Plasmid

    #238257

    Purpose

    For overexpression of EGFP-control

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD-Citron1

    Plasmid

    #134300

    Purpose

    Bacterial expression of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Use

    Tags

    6-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPR v2-sgControl

    Plasmid

    #230080

    Purpose

    Crispr knock out control

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    non-specific sequence control
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. control (pGS701)

    Plasmid

    #160037

    Purpose

    pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRetroX-TRE3G_NINJ1

    Plasmid

    #201181

    Purpose

    Dox-inducible expression of NINJ1 gene in mammalian cells

    Depositor

    Jonathan Kagan

    Article

    Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

    Insert

    Ninjurin 1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. 60-p3E_INTRON-EmptyRNAi

    Plasmid

    #163380

    Purpose

    p3E-Vector (Gateway R2-L3) for Tol2-kit cloning. Vector for performing RNAi (gene silencing - knockdown) in zebrafish via cloning and expression of synthetic miR(s).

    Depositor

    Jean Giacomotto

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge