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Showing: 1181 - 1200 of 1245 results

  3. cfmTurquoise-N

    Plasmid
    #139284
    Purpose
    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.
    Depositor
    Ikuo Wada
    Article
    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d
    Insert
    cfmTurquoise
    Use
    Tags
    Multi-cloning sites
    Expression
    Mammalian
    Mutation
    Cys48Ser and Cys70/Met
    Promoter
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    139284-plasmid-map-sequence-id-291944

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  6. pQE60-mPU.1-ETS

    Plasmid
    #85733
    Purpose
    Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4
    Depositor
    Gregory Poon
    Article
    Poon et al J Biol Chem. 2012 May 25;287(22):18297-3
    Insert
    ETS domain of transcription factor PU.1 (Spi1 Mouse)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    85733-plasmid-map-sequence-id-175402

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  7. mCit-Snap25b D179K A199R

    Plasmid
    #53259
    Purpose
    SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    SNAP-25B (Snap25 Rat)
    Use
    Tags
    4aa-linker and mCitrine
    Expression
    Mammalian
    Mutation
    D179K and A199R
    Promoter
    CMV
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  15. pSLQ10878

    Plasmid
    #183964
    Purpose
    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183964-plasmid-map-sequence-id-363120

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  20. pCS-CG-WT-Cer-VWF

    Plasmid
    #124827
    Purpose
    Expresses multimeric human VWF-ECFP fusion protein. The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.
    Depositor
    Sriram Neelamegham
    Article
    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:
    Insert
    VWF with cyan fluorescent protein fusion between D'D3 and A1 domains (VWF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124827-plasmid-map-sequence-id-243867

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Showing: 1181 - 1200 of 1245 results