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Showing: 1181 - 1200 of 15918 results

  1. pCEP4-SERTR464Q-myc

    Plasmid
    #107468
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R464Q approximately wildtype transport ability
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R464Q
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107468-plasmid-map-sequence-id-205588

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  2. pCEP4-SERTT439M-myc

    Plasmid
    #107466
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T439M, decreased surface expression and transport relative to wildtype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T439M
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107466-plasmid-map-sequence-id-205583

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  3. pCEP4-SERTI425L-myc

    Plasmid
    #107465
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425L conferring approximately wildtype transport phenotype, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    I425L
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107465-plasmid-map-sequence-id-205576

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  5. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

    Plasmid
    #52898
    Purpose
    YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Ypd*1-L-IFP-F[2]
    Use
    Tags
    L-IFP-F[2]
    Expression
    Yeast
    Mutation
    Changed Tryptophane 80 to Alanine
    Promoter
    GAL1
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pC5Kan-P2A

    Plasmid
    #51814
    Purpose
    P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message
    Depositor
    Barry Ganetzky
    Article
    Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:
    Insert
    P2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)
    Use
    Shuttle vector for insect expression vectors
    Tags
    Expression
    Mutation
    codons altered for Drosophila and to eliminate re…
    Promoter
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    51814-plasmid-map-sequence-id-79554

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  10. pNvT-MHC::mCh

    Plasmid
    #67943
    Purpose
    Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI
    Depositor
    Ulrich Technau
    Article
    Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107
    Insert
    mCherry
    Use
    Transgenic reporter in the sea anemone, nematoste…
    Tags
    Expression
    Mutation
    Promoter
    Myosin Heavy Chain1 (MyHC1)
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67943-plasmid-map-sequence-id-266110

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  11. pDule2-Mb haloTyrRS C6

    Plasmid
    #160378
    Purpose
    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.
    Depositor
    Ryan Mehl
    Article
    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574
    Insert
    C6 HaloTyrosine tRNA synthatase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    L270S, Y271L, N311G, C313T
    Promoter
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    160378-plasmid-map-sequence-id-315318

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  15. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Use
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    176151-plasmid-map-sequence-id-356068

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  16. pHDR-USR-SpCas9

    Plasmid
    #208829
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    208829-plasmid-map-sequence-id-419661

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  18. pDule-Mb haloTyrRS C6

    Plasmid
    #160377
    Purpose
    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.
    Depositor
    Ryan Mehl
    Article
    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574
    Insert
    C6 HaloTyrosine tRNA synthatase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    L270S, Y271L, N311G, C313T
    Promoter
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    160377-plasmid-map-sequence-id-315316

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  19. BtuF

    Plasmid
    #92209
    Purpose
    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter
    Depositor
    Douglas Rees
    Article
    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99
    Insert
    BtuF (btuF )
    Use
    Tags
    6x His Tag and PelB Signal Sequence
    Expression
    Bacterial
    Mutation
    BtuF signal sequence removed and uses the PelB si…
    Promoter
    T7 Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92209-plasmid-map-sequence-id-183562

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Showing: 1181 - 1200 of 15918 results