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Showing: 1181 - 1200 of 9691 results

  1. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  2. pGTRm

    Plasmid
    #127197
    Purpose
    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences
    Depositor
    Jordan Green
    Article
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Insert
    tRNA-gRNA PCR template
    Use
    Template plasmid for generation of golden gate pc…
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127197-plasmid-map-sequence-id-248423

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  6. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid
    #115344
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
    Depositor
    Steven Jacobsen
    Insert
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    115344-plasmid-map-sequence-id-222905

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  8. p-dCas9-LSD1-K661A-Hygro

    Plasmid
    #104414
    Purpose
    transient expression of dCas9-dLSD1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dLSD1 (KDM1A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104414-plasmid-map-sequence-id-204405

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  9. pTFE3-donor

    Plasmid
    #112377
    Purpose
    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP
    Depositor
    Kevin White
    Insert
    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112377-plasmid-map-sequence-id-229742

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  11. pMLX-donor

    Plasmid
    #112370
    Purpose
    CRISPR donor plasmid to tag human transcription factor MLX with GFP
    Depositor
    Kevin White
    Insert
    MLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:17:42570798:G>A, hg38:17:42570950:T>G,…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112370-plasmid-map-sequence-id-229723

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  12. pTHRB-donor

    Plasmid
    #112333
    Purpose
    CRISPR donor plasmid to tag human transcription factor THRB with GFP
    Depositor
    Kevin White
    Insert
    THRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112333-plasmid-map-sequence-id-228563

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  13. pZNF445-donor

    Plasmid
    #112332
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF445 with GFP
    Depositor
    Kevin White
    Insert
    ZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs78585428, hg38:3:44446218:C>T, hg38:3:444466…
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112332-plasmid-map-sequence-id-228561

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  14. pZNF785-donor

    Plasmid
    #112317
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF785 with GFP
    Depositor
    Kevin White
    Insert
    ZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:16:30582292:A>T, hg38:16:30582302:A>T,…
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112317-plasmid-map-sequence-id-228521

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  19. qTAG-N-Solo-mStayGold-TJP1

    Plasmid
    #227300
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227300-plasmid-map-sequence-id-451538

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  20. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

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Showing: 1181 - 1200 of 9691 results