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pLenti.PGK.H2B-chFP.W

Plasmid

#51007

Purpose

Lentivector that expresses H2B-chFP from internal human PGK promoter

Depositor

Rusty Lansford

Article

Huss et al Development. 2015 Jul 24. pii: dev.12139

Insert

H2B-ChFP

Use

Lentiviral

Tags

histone2B

Expression

Mammalian

Promoter

human PGK

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-RNF8

Plasmid

#99396

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF8

Depositor

Junjie Chen, Michael Huen

Article

Huen et al Cell. 2007 Nov 30;131(5):901-14. Epub 20

Insert

RNF8 (RNF8 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pIRESpuro-Flag-Pol b

Plasmid

#23257

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Insert

DNA polymerase beta (POLB Human)

Tags

Flag

Expression

Mammalian

Available Since

May 13, 2010

Availability

Academic Institutions and Nonprofits only
AIMTOR A757

Plasmid

#140829

Purpose

AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

Depositor

Anne Bonnieu

Article

Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

Insert

AIMTOR A757 (ULK1 Human)

Tags

NES Nuclear export signal

Expression

Mammalian

Mutation

The insert comprises only a small sequence of hum…

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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Daxx/pEGFP

Plasmid

#119021

Purpose

Express GFP-Daxx in mamalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

death-domain associated protein (Daxx) (DAXX Human)

Tags

GFP

Expression

Mammalian

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLBS-mScarlet

Plasmid

#129337

Purpose

cytosol marker, lentivirus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

mScarlet (Addgene 85042)

Use

Lentiviral

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-pm-iATPSnFR1.0

Plasmid

#102552

Purpose

expresses membrane displayed ATP sensor in astrocytes

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

Plasmid

#164611

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Expression

Mammalian

Mutation

S383T

Promoter

CMV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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JT113-pETDuet1-(R)-hREV3L

Plasmid

#64872

Purpose

bacterial expression of human REV3L

Depositor

Richard Wood

Article

Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

Insert

REV3L (REV3L Human)

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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HHM-iATPSnFR1.0

Plasmid

#102546

Purpose

expresses ATP sensor in bacteria

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Expression

Bacterial

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Sstr3-GFP-DEST

Plasmid

#49098

Purpose

Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Sstr3 (Sstr3 Mouse)

Tags

GFP and acceptor peptide (AP)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LumiScarlet

Plasmid

#126623

Purpose

A BRET-based mScarlet-I-LumiLuc fusion for red-shifted bioluminescence emission

Depositor

Huiwang Ai

Article

Yeh et al ACS Chem Biol. 2019 Apr 17. doi: 10.1021

Insert

LumiScarlet

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSCAC-69

Plasmid

#31241

Purpose

Mitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafish

Depositor

Seok-Yong Choi

Article

Kim et al Biotechniques. 2008 Sep . 45(3):331-4.

Insert

mitochondrial localization enhanced green fluorescent protein

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET iGluSnFR3 v857

Plasmid

#175186

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

iGluSnFR3 v857

Use

Synthetic Biology

Tags

His tag, T7 leader, Xpress epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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VAMP3-mCherry

Plasmid

#92423

Purpose

Expression of VAMP3 C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBIS-GALkFLP(URA)

Plasmid

#49460

Purpose

overexpression of Flp step-arrest mutant in yeast, URA selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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MGAT2-RpHLuorin2

Plasmid

#171718

Purpose

Cis-/medial-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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EKARet-cyto

Plasmid

#96895

Purpose

FLIM sensor for ERK activation in cytosol

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

FLIM Sensor for ERK Activity in Cytosol

Tags

EGFP and sREAChet

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR

Plasmid

#112175

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-ZMYND11

Plasmid

#65402

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ZMYND11 (ZMYND11 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only