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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,786 results for: golden gate

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Showing: 1201 - 1220 of 6786 results

  1. T17m

    Plasmid
    #121036
    Purpose
    MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121036-plasmid-map-sequence-id-245428

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  2. T12m

    Plasmid
    #121031
    Purpose
    MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120029600 (strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121031-plasmid-map-sequence-id-249041

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  3. C115m

    Plasmid
    #121014
    Purpose
    MoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TP901 integrase
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121014-plasmid-map-sequence-id-236321

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  4. P18m

    Plasmid
    #120941
    Purpose
    MoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120941-plasmid-map-sequence-id-235947

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  5. P57m

    Plasmid
    #120949
    Purpose
    MoClo golden gate assembly AB part for pCin* (Adam Meyer's improved pCin promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pCin Adam Meyer
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120949-plasmid-map-sequence-id-235961

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  6. U25m

    Plasmid
    #121027
    Purpose
    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RiboJ + UTR1
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121027-plasmid-map-sequence-id-238073

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  8. pAV114

    Plasmid
    #63181
    Purpose
    pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63181-plasmid-map-sequence-id-104885

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  9. T18m

    Plasmid
    #121037
    Purpose
    MoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S2P21 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121037-plasmid-map-sequence-id-236351

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  10. C92m

    Plasmid
    #121000
    Purpose
    MoClo golden gate assembly CD part for sfCFP-pdt3 (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with a pdt3 tag for mfLon protease).
    Depositor
    Richard Murray
    Insert
    sfCFPpdt3 (Chen,Bennett)
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121000-plasmid-map-sequence-id-238058

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  11. C101m

    Plasmid
    #121005
    Purpose
    MoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).
    Depositor
    Richard Murray
    Insert
    sfCFP with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121005-plasmid-map-sequence-id-236158

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  12. P1m

    Plasmid
    #120934
    Purpose
    MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.
    Depositor
    Richard Murray
    Insert
    J23101
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120934-plasmid-map-sequence-id-235942

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  13. C86m

    Plasmid
    #120997
    Purpose
    MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    X174E
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120997-plasmid-map-sequence-id-236126

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  14. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

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Showing: 1201 - 1220 of 6786 results