We narrowed to 2,178 results for: ipp

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Dual-sgRNA_hU6-mU6

Plasmid

#154194

Purpose

Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-hMLH1dn for IVT

Plasmid

#178114

Purpose

Template for in vitro transcription of human MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized) (MLH1 Human)

Use

Template for in vitro transcription

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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HIP-flash (HOP-flash mutant)

Plasmid

#83466

Purpose

Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)

Use

Luciferase

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-flag YAP2 5SA

Plasmid

#27371

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP2 (YAP1 Human)

Tags

flag

Expression

Mammalian

Mutation

Serines at positions 61, 109, 127, 128, 131, 163,…

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV2-SEP-GluA1 (M1)

Plasmid

#64942

Purpose

mammalian expression of rat GluA1

Depositor

Jeremy Henley

Article

Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

Insert

GluA1 (Gria1 Rat)

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_FLAG-YAP1

Plasmid

#173001

Purpose

constitutive expression of FLAG-tagged YAP1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

FLAG-YAP1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-FLAG-YFP-KIBRA

Plasmid

#235683

Purpose

Express YFP tagged KIBRA gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

KIBRA (WWC1 Human)

Use

Lentiviral

Tags

FLAG-YFP

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-5SA

Plasmid

#33093

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Promoter

CMV/MSV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pSN672

Plasmid

#177362

Purpose

Expresses human KIF1A(1-393) fused with leucine zipper and His tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Tags

His tag and Leucine zipper

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX NPC1-FLAG-TiD

Plasmid

#199748

Purpose

Lentiviral construct to stably express NPC1 TurboID

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

NPC1 (NPC1 Human)

Tags

Flag and TurboID

Expression

Mammalian

Promoter

EF1a

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-NF2-CFP

Plasmid

#235687

Purpose

Express CFP tagged NF2 gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

NF2 (CA12 Human)

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC1

Plasmid

#172994

Purpose

constitutive expression of FLAG-tagged WWC1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC1 (WWC1 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FDIO-ArchT-GFP

Plasmid

#124640

Purpose

AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

ArchT

Use

AAV; Flp/frt

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7 EGFPC2 TAZ

Plasmid

#66850

Purpose

Express GFP-fused TAZ by lentivirus

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

WWTR1/TAZ (WWTR1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-LATS2

Plasmid

#172987

Purpose

constitutive expression of FLAG-tagged LATS2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-LATS2 (LATS2 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLP17_Lenti α-syn

Plasmid

#239419

Purpose

Lentiviral plasmid for SpCas9-based CRISPR KO of mouse α-syn

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

mouse a-synuclein sgRNA (Snca Mouse)

Use

Lentiviral

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-S94A

Plasmid

#33094

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S94A

Promoter

CMV/MSV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pQCXIH-Flag-YAP-S127A

Plasmid

#33092

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

S127A

Promoter

CMV/MSV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCOCK CMVwt

Plasmid

#46559

Purpose

Mammalian expression plasmid with EGFP expressed from CMV wild-type promoter.

Depositor

Clifford Wang

Article

Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

Insert

CMV wt promoter

Use

Synthetic Biology

Expression

Mammalian

Mutation

Our version has a G at base 724 (reference has C)

Promoter

CMV

Available Since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only

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