We narrowed to 9,552 results for: FIE

1. pRS315Nab3

   Plasmid

   #110532

   Purpose

   Expresses yeast NAB3 using the endogenous promoter

   Depositor

   Daniel Reines

   Article

   Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

   Insert

   NAB3 (NAB3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-CaaX(tH)

   Plasmid

   #109539

   Purpose

   Multisite gateway vector for 3' tagging with the CaaX domain from Hras

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   CaaX domain from Hras
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPP378

   Plasmid

   #158797

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-1 (D371A)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

   Plasmid

   #196710

   Purpose

   Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

   Plasmid

   #52478

   Purpose

   Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

   Depositor

   Sebastian Seung

   Article

   Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

   Inserts

   EGFP
   nuclear mTagBFP
   Synaptophysin-TagRFP-T
   

   Use

   Rabies virus

   Tags

   TagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPP384

   Plasmid

   #158799

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-3 (D413A)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Syn-ChR-tdTomato

   Plasmid

   #99843

   Purpose

   AAV expression of ChR2(H134R)-tdTomato under the Syn promoter

   Depositor

   Gordon Shepherd

   Insert

   ChR2(H134R)-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   ChR2(H134R), carrying a single point mutation at …

   Promoter

   human synapsin I promoter

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p1148-pCSDest-AcrIIA4-mCherry

   Plasmid

   #126204

   Purpose

   Mammalian expression of AcrIIA4 fused to mCherry

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Insert

   codon-optimized AcrIIA4
   

   Use

   Tags

   FLAG/NLS and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC-hTDG-PreE2

   Plasmid

   #52272

   Purpose

   Bacterial expression of human TDG C-terminally fused to SUMO-E2 and GST

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   hTDG (TDG Human)
   

   Use

   Tags

   Ubc9-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET_His6-MBP-TEV-Cas2

    Plasmid

    #186275

    Purpose

    Expresses Thiomicrospira Cas2 in E. coli

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicropira Cas2
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ1656

    Plasmid

    #51483

    Purpose

    miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Setd3-Flag-pGEX4t-1

    Plasmid

    #49145

    Purpose

    bacterial expression of GST-Setd3-Flag

    Depositor

    Jing H Wang

    Article

    Chen et al Haematologica. 2013 May;98(5):739-43. do

    Insert

    Setd3 (Setd3 Mouse)
    

    Use

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-3a-His-MBP-TEV-VC_Cas1_WT

    Plasmid

    #120364

    Purpose

    Expresses wildtype V-C Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VC_Cas1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-3a-His-MBP-TEV-VD_Cas1_WT

    Plasmid

    #120365

    Purpose

    Expresses wildtype V-D Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VD_Cas1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVC-CRISPR1

    Plasmid

    #120366

    Purpose

    Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    CRISPR1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVC-CRISPR

    Plasmid

    #120369

    Purpose

    Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Inserts

    CRISPR1
    CRISPR2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVD_CRISPR

    Plasmid

    #120370

    Purpose

    Plasmid containing shortened version of V-D CRISPR array for integration assays

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VD_CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p6661 MSCV-IP N-HA only 16E6

    Plasmid

    #42603

    Depositor

    Peter Howley

    Article

    White et al J Virol. 2012 Dec;86(24):13174-86. doi:

    Insert

    HPV16 E6
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

19. p144 pSV-EGFP/beta-gal

    Plasmid

    #8386

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    floxed EGFP
    

    Use

    Cre/Lox; Beta-gal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pJMC-2-Reverse

    Plasmid

    #197801

    Purpose

    Level 1 Module 2 destination vector (reverse orientation MoClo insert)

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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