Addgene

We narrowed to 9,378 results for: FIE

Showing: 1221 - 1240 of 9378 results

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p1148-pCSDest-AcrIIA4-mCherry

Plasmid

#126204

Purpose

Mammalian expression of AcrIIA4 fused to mCherry

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIA4

Use

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Syn-ChR-tdTomato

Plasmid

#99843

Purpose

AAV expression of ChR2(H134R)-tdTomato under the Syn promoter

Depositor

Gordon Shepherd

Insert

ChR2(H134R)-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

ChR2(H134R), carrying a single point mutation at …

Promoter

human synapsin I promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VC_Cas1_WT

Plasmid

#120364

Purpose

Expresses wildtype V-C Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VC_Cas1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VD_Cas1_WT

Plasmid

#120365

Purpose

Expresses wildtype V-D Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VD_Cas1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pVC-CRISPR1

Plasmid

#120366

Purpose

Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

CRISPR1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pVC-CRISPR

Plasmid

#120369

Purpose

Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Inserts

CRISPR1
CRISPR2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pVD_CRISPR

Plasmid

#120370

Purpose

Plasmid containing shortened version of V-D CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VD_CRISPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSC-hTDG-PreE2

Plasmid

#52272

Purpose

Bacterial expression of human TDG C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Use

Tags

Ubc9-GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pJMC-2-Reverse

Plasmid

#197801

Purpose

Level 1 Module 2 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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Setd3-Flag-pGEX4t-1

Plasmid

#49145

Purpose

bacterial expression of GST-Setd3-Flag

Depositor

Jing H Wang

Article

Chen et al Haematologica. 2013 May;98(5):739-43. do

Insert

Setd3 (Setd3 Mouse)

Use

Tags

FLAG and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ1656

Plasmid

#51483

Purpose

miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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p6661 MSCV-IP N-HA only 16E6

Plasmid

#42603

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
p144 pSV-EGFP/beta-gal

Plasmid

#8386

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox; Beta-gal

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
lentiCRISPRv2-7SK-Blast

Plasmid

#183620

Purpose

lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

Depositor

Bill Sugden

Article

Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Modified 7SK

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pI12

Plasmid

#24273

Depositor

Mariano Garcia-Blanco

Article

Baraniak et al Mol Cell Biol. 2003 Dec . 23(24):9327-37

Insert

modified adenovirus exons and introns

Use

T7 for in vitro transcription

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2010

Availability

Academic Institutions and Nonprofits only

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p169 pCMV/betaAc-EGFP/beta-gal

Plasmid

#8388

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP beta-gal

Use

Cre/Lox; Beta-gal

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pCFJ1258

Plasmid

#44478

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-p85α_wt

Plasmid

#39496

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 (PIK3R1 Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pF3-CAG-Tol-FRT

Plasmid

#141349

Purpose

Shuttling cassette containing the Tol ORF

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

Tol cassette

Use

Mouse Targeting

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pME-myristo

Plasmid

#109541

Purpose

Multisite gateway vector for 5' tagging with the 5' myristoylation sequence from ARF5

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

5' myristoylation sequence from ARF5

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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