We narrowed to 18,031 results for: Mut

1. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1x

   Plasmid

   #182253

   Purpose

   Human PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PDAP1 gene mutated in 1 binding site for mir-150
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3x

   Plasmid

   #182255

   Purpose

   Human PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PDAP1 gene mutated in 3 binding sites for mir-150
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-Hygro-Ef1A-Myc-POLB(PAMmut)

   Plasmid

   #176150

   Purpose

   N-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Mutation in the PAM site used by POLB gRNA1

   Promoter

   EF1A

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmirGlo-RBM8A-3'UTRAluY-Sz mut 2598-4066

   Plasmid

   #171135

   Purpose

   reporter

   Depositor

   Emanuela Felley-Bosco

   Article

   Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

   Insert

   RBM8A 3'UTR (RBM8A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A3302G, A3356G, A3373G, A3389G,A3451G, T3729C, A3…

   Promoter

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

   Plasmid

   #173167

   Purpose

   Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
   

   Use

   Retroviral

   Tags

   3XFLAG

   Expression

   Mutation

   Synonymous mutations at sgRNA sites, G2A, S6A

   Promoter

   MMLV

   Available Since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5E-161K enhancer-ETS-CORE-MUT

   Plasmid

   #138372

   Purpose

   Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   human 161K human enhancer with ETS bind sites mutated
   

   Use

   Gateway compatible

   Tags

   Expression

   Mutation

   ETS factor binding sites mutated

   Promoter

   NONE

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. dre-cdk2 3'UTR sicheck2 199 mut

   Plasmid

   #121122

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   miR-199 seed binding site mutated

   Promoter

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Fibronectin-human-mutated N-terminal (N) O-Glycosylation site

   Plasmid

   #120404

   Purpose

   enables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serine

   Depositor

   Inaam Nakchbandi

   Article

   Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.

   Insert

   Fibronectin (FN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Containing complete variable region with a mutati…

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

   Plasmid

   #117663

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Benjamin Glick, Pau Ferrer

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pOst1-pro-af(MUT2)-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLex-FLAG-FASTKD4 mutant delta 226

    Plasmid

    #73621

    Purpose

    Expression vector containing FASTKD4 mutant delta 226

    Depositor

    Vamsi Mootha

    Article

    Wolf et al Cell Rep. 2014 May 8;7(3):918-31. doi: 1

    Insert

    FASTKD4 (TBRG4 Human)
    

    Use

    Tags

    Expression

    Mutation

    Y616A, L617A, K618A, K620A

    Promoter

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1287 p3E-mut let-7 etv2 3’ UTR

    Plasmid

    #49011

    Purpose

    Zebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Gateway 3' entry

    Tags

    Expression

    Mutation

    Mutated the Let7 miRNA binding sites

    Promoter

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pmyc-GFP-TNRC6A-del.GW-II-NES-mut

    Plasmid

    #42038

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    deleted aa716-772, F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmyc-GFP-TNRC6A-del.GW-I-NES-mut

    Plasmid

    #42037

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    deleted aa457-532, F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmyc-GFP-TNRC6A-del.GW-I+II-NES-mut

    Plasmid

    #42039

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    deleted aa457-532 and aa716-772, F951A, F955A, I9…

    Promoter

    CMV

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. CJ64 (miR-1003 5' splice site mut)

    Plasmid

    #17785

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    dme-miR-1003 minigene 5' splice mut
    

    Use

    Drosophila p-element vector

    Tags

    Expression

    Mutation

    5' splice site mut + compsensatory stem

    Promoter

    Available Since

    May 12, 2008

    Availability

    Academic Institutions and Nonprofits only

16. CJ45 (miR-1003 5' splice site mut)

    Plasmid

    #17775

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    dme-miR-1003 minigene 5' splice mut
    

    Use

    Drosophila metallothionein gene promoter drives e…

    Tags

    Expression

    Insect

    Mutation

    5' splice site mut + compsensatory stem

    Promoter

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

17. 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

    Plasmid

    #209294

    Purpose

    mitoBE expression vector, target mtRNR2 gene.

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

    Insert

    SOD2-3xHA-TALE(RNR2-Left)-MutH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid

    #176279

    Purpose

    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    GYG to AAA (aa144-146)

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mammalian expression: Z-lock cofilin dark state mutant

    Plasmid

    #141136

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-C1 SNX9 RYK mutant R286A Y287A K288A

    Plasmid

    #161993

    Purpose

    Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    SNX9 (Snx9 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R286A Y287A K288A

    Promoter

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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