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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,329 results for: nes

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Showing: 1221 - 1240 of 22329 results

  2. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    100901-plasmid-map-sequence-id-197197

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  7. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Publication
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    83890-plasmid-map-sequence-id-240902

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  10. T4 Lysozyme WT*

    Plasmid
    #18111
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Expression
    Bacterial
    Mutation
    No cysteines: C54T, C97A
    Available since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    18111-plasmid-map-sequence-id-174143

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  13. pTP1172

    Plasmid
    #104161
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)
    Depositor
    Dirk Görlich
    Publication
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    104161-plasmid-map-sequence-id-200072

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  14. pRK5-EGFP-Tau E14

    Plasmid
    #46907
    Purpose
    expresses EGFP tagged Tau E14 in mammalian cells
    Depositor
    Karen Ashe
    Publication
    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tau E14 (MAPT Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    all 14 S/P or T/P amino acid residues (T111, T153…
    Promoter
    CMV
    Available since
    July 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    46907-plasmid-map-sequence-id-364531

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Showing: 1221 - 1240 of 22329 results