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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,435 results for: 5-Oct

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Showing: 1241 - 1260 of 1435 results

  1. eNS2B47NS3 S135A

    Plasmid
    #155318
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    S135A
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155318-plasmid-map-sequence-id-311249

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  2. CIRTS-FTO-control

    Plasmid
    #216859
    Purpose
    CIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-FTO-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    mCherry2
    Expression
    Mammalian
    Promoter
    Syn1, U6
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216859-plasmid-map-sequence-id-430696

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  3. pMRX-IBU-pSu9-HaloTag7-SNAP

    Plasmid
    #217319
    Purpose
    Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.
    Depositor
    Noboru Mizushima
    Article
    Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:
    Insert
    Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
    Use
    Retroviral
    Tags
    HaloTag and SNAP
    Promoter
    CMV
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    217319-plasmid-map-sequence-id-436845

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  4. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (AAV5)

    Viral Prep
    #202199-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (#202199). In addition to the viral particles, you will also receive purified pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE plasmid DNA. Synapsin-driven, Cre-dependent expression of bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized opsin PdCO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  6. pCDF-BirA

    Plasmid
    #250993
    Purpose
    Vector for co-expression of BirA for in-vivo biotinylation of Avi-tagged proteins. Spectinomycin-resistant, compatible with pET and pACYC vectors. Expression of BirA is driven by T7/Lac promoter.
    Depositor
    Opher Gileadi
    Article
    Keates et al N Biotechnol. 2012 Jun 15;29(5):515-25.
    Insert
    BirA (birA )
    Expression
    Bacterial
    Available Since
    June 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    250993-plasmid-map-sequence-id-511671

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  7. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23101 and VanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166978-plasmid-map-sequence-id-331328

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  14. G protein alpha-o-YFP

    Plasmid
    #55776
    Purpose
    This G protein alpha-o construct contains internal insertions of YFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    G protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
    Tags
    EE epitope was introduced internally with D167E a…
    Expression
    Mammalian
    Mutation
    A BglII site was removed from the 5' untrans…
    Promoter
    CMV
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55776-plasmid-map-sequence-id-112541

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Showing: 1241 - 1260 of 1435 results