We narrowed to 2,475 results for: T2A

1. vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)

   Plasmid

   #160044

   Purpose

   For expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA)

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Insert

   iaaH (iaaH )
   

   Use

   Synthetic Biology

   Tags

   3xNLS-Citrine

   Expression

   Mammalian

   Promoter

   pEF1s

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

   Plasmid

   #64220

   Purpose

   Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

   Plasmid

   #64219

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E1B

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP

   Plasmid

   #228359

   Purpose

   RfxCas13d (NLS-RfxCas13d-NLS) with 2A-EGFP and 2A-mCD4 for RNA knockdown

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Insert

   RfxCas13d
   

   Use

   Lentiviral

   Tags

   2A-EGFP, 2A-mCD4, HA, and NLS

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP

   Plasmid

   #196988

   Purpose

   Derived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vector

   Depositor

   Elizabeth Kirby

   Article

   Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

   Insert

   Nestin-dCas9-KRAB-T2a-GFP
   

   Use

   CRISPR and Lentiviral

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR

   Plasmid

   #160042

   Purpose

   For the auxin receiver that report on auxin.

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Inserts

   osTIR1
   BlastR
   

   Use

   Luciferase

   Tags

   mAID and mCherry

   Expression

   Mammalian

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101103

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   VEGFR1-dCas9(C)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69546

   Purpose

   Tet-On system expressing MyoD-2A-GFP-2xNLS.

   Depositor

   Charles Gersbach

   Inserts

   eGFP
   Myod1 (Myod1 Mouse)
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Mutation

   Removed stop codon to fuse it to the 2A-eGFPnls i…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

   Plasmid

   #199457

   Purpose

   Expression of dCas13-VPR in mammalian cells

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   dCas13
   

   Use

   Lentiviral

   Tags

   VPR-T2A-HaloTag

   Expression

   Mammalian

   Mutation

   Nuclease-dead H133A and H1058A

   Promoter

   CAG

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

    Plasmid

    #199198

    Purpose

    Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int-G418R
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn T21R-F8.2 (S54L+T127A)-T2A-mCherry

    Plasmid

    #225590

    Purpose

    AAV transgene plasmid with hSyn promoter for expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-F8.2 (S54L+T127A)-T2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216249

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgRANGAP.C1 (RANGAP1 Human)
    

    Use

    CRISPR; Endogenous tagging

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo

    Plasmid

    #78353

    Purpose

    Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Promoter

    pEF alpha

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

    Plasmid

    #78352

    Purpose

    Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-KRAB
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Promoter

    pEF alpha

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

    Plasmid

    #195543

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

    Plasmid

    #186968

    Purpose

    Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
    

    Use

    Lentiviral

    Tags

    rTetR fusion

    Expression

    Mammalian

    Promoter

    EF-1alpha promoter

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid

    #108362

    Purpose

    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

    Depositor

    Luisa Cochella

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Inserts

    Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    

    Tags

    2xFlag, MYC, T2A peptide, and TY1 linker

    Expression

    Worm

    Promoter

    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

    Plasmid

    #114726

    Purpose

    dCas9-VP160 expression in mammalian cells

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    dCas9-VP160
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP

    Plasmid

    #194934

    Purpose

    LV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCA24-pHR-CMV-stdMCP-p65-HSF1-T2A-GFP

    Plasmid

    #199455

    Purpose

    Expression of stdMCP-PH in mammalian cells

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    stdMCP-PH
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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