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pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

Plasmid

#162280

Purpose

Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Publication

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN8A Variant 1, stabilized (SCN8A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

Modified human HSPA5 intron 4 inserted after bp23…

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV5 HA-TBRII (delta Cyt)

Plasmid

#14051

Depositor

Joan Massague

Publication

Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

Insert

TGF beta rec II (delta cyt) (TGFBR2 Human)

Tags

HA (PYDVPDYA)

Expression

Mammalian

Mutation

Dominant negative mutant. A stop codon was introd…

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158653

Purpose

making transgenics expressing a UAS driven mCherry and NTR 2.0

Depositor

Jeff Mumm

Publication

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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dClover2-C1

Plasmid

#54577

Purpose

Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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pMDC123SB-AtMIR390a-B/c

Plasmid

#51775

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

Depositor

James Carrington

Publication

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtMIR390a-B/c

Use

Plant expression

Mutation

A. thaliana MIR390a precursor sequence including …

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTriEx-PA-Rac1

Plasmid

#22024

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6X His

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pRSET-mEosEM

Plasmid

#132708

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Publication

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mEosEM

Expression

Bacterial

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAGM4723

Plasmid

#48015

Purpose

Please see depositor comments section.

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

30 Articles

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pKHR5

Plasmid

#74593

Purpose

an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.

Depositor

David Grunwald

Publication

Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

Insert

FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette

Use

Cre/Lox; Zebrafish expression

Promoter

alpha-crystallin

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICSL11024 (pICH47732::NOSp-NPTII-OCST)

Plasmid

#51144

Purpose

Confers KAN resistance in planta

Depositor

Jonathan D Jones

Insert

NOSp-NPTII-OCST

Available since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pIRES proHB-EGF WT

Plasmid

#11602

Depositor

Rosalyn Adam

Publication

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Expression

Mammalian

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
PR_GEF (2XPDZ-mCherry-Larg (DH)

Plasmid

#80407

Purpose

Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Publication

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DH domain only (aa 766-997)

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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PL-SIN-PGK-EiP

Plasmid

#21312

Purpose

Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance

Depositor

James Ellis

Publication

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HA-E2F-1 wt-pRcCMV

Plasmid

#21667

Depositor

William Kaelin

Publication

Krek et al Cell. 1994 Jul 15. 78(1):161-72.

Insert

E2F-1 (E2F1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wild type-

Available since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pDONR223-RAF1

Plasmid

#23832

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RAF1 (RAF1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pEGFP-C1X-Nup93

Plasmid

#87336

Purpose

To express human Nup93 in mammalian cells

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLARRY-EGFP

Plasmid

#140025

Purpose

Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

Depositor

Fernando Camargo

Publication

Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

Insert

EGFP

Use

Lentiviral

Promoter

EF1alpha

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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AAVS1 pegRNA2 with trimmed attP

Plasmid

#222347

Purpose

pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

AAVS1 attP-installation pegRNA2

Use

CRISPR

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Mutation

R175H

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Publication

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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