We narrowed to 2,423 results for: MEA;

1. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #187324

   Purpose

   Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLED.GluA2

   Plasmid

   #193017

   Purpose

   GluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic reporter (EGFP and dsRed-Express2)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Venus-tBid-4E-pEGFP-C1

   Plasmid

   #166743

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   tBid-4E (Bid Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   4 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10 KRAB-T2A-mCherry-BSD-WPRE

   Plasmid

   #188757

   Purpose

   Lentiviral backbone containing ZNF10 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   ZNF10 KRAB (ZNF10 Human)
   

   Use

   Lentiviral

   Tags

   rTetR(SE-G72P)-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLED.ENS

   Plasmid

   #193016

   Purpose

   Excitatory neuron-specific reporter (hSyn promoter, Synrg exon, bichromatic reporter)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic reporter (EGFP and dsRed-Express2)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394

   Plasmid

   #128387

   Purpose

   Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption

   Depositor

   Craig Duvall

   Article

   Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

   Inserts

   NLuc398-3xGGGGS-LGALS8 (LGALS8 Synthetic, Human)
   CALCOCO2-3xGGGGS-CLuc394 (CALCOCO2 Human)
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   CLuc394 and NLuc398

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and IRES

   Available since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SEPT_Cdk2-T160A-siR

   Plasmid

   #73975

   Purpose

   HDR template to generate Cdk2-T160A-siR

   Depositor

   Manuel Kaulich, Steven Dowdy

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160A (CDK2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   T160 mutated to Ala and siRNA target site mutated

   Promoter

   Available since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMV9 lenti pEF-rTetR(SE-G72P)-3XFLAG-NOTC2(Notch)-T2A-mCherry-BSD-WPRE

   Plasmid

   #188755

   Purpose

   Lentiviral backbone containing NOTC2 Notch domain as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   NOTC2 Notch (NOTCH2 Human)
   

   Use

   Lentiviral

   Tags

   rTetR(SE-G72P)-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-SEPT_Cdk2-T160E-siR

   Plasmid

   #73976

   Purpose

   HDR template to generate Cdk2-T160E-siR

   Depositor

   Manuel Kaulich, Steven Dowdy

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160E (CDK2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   T160 mutated to Glu and siRNA target site mutated

   Promoter

   Available since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCRISPR v2-sgCrls1-1#

    Plasmid

    #234765

    Purpose

    knockout Crls1

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Crls1 (Crls1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiCRISPR v2-sgCrls1-2#

    Plasmid

    #234766

    Purpose

    knockout Crls

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Crls1 (Crls1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lentiCRISPR v2-sgPlscr3-2#

    Plasmid

    #234772

    Purpose

    knockout Plscr3

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Plscr3 (Plscr3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiCRISPR v2-sgPlscr3-1#

    Plasmid

    #234767

    Purpose

    knockout Plscr3

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Plscr3 (Plscr3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMV10 lenti pEF-rTetR(SE-G72P)-3XFLAG-WWP2(WW)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188756

    Purpose

    Lentiviral backbone containing WWP2 WW domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    WWP2 WW (WWP2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PJT220 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF473 KRAB-T2A-mCherry-BSD-WPRE

    Plasmid

    #188759

    Purpose

    Lentiviral backbone containing ZNF473 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    ZNF473 KRAB (ZNF473 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMV2 AAV-RapaInducible-NFZ-HGF

    Plasmid

    #188749

    Purpose

    Rapamycin inducible AAV vector expressing HGF

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    HGF (HGF Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Rapamycin inducible

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMV8 lenti pEF-rTetR(SE-G72P)-3XFLAG-PYGO1(PHD)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188754

    Purpose

    Lentiviral backbone containing PYGO1 PHD domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    PYGO1 PHD (PYGO1 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188743

    Purpose

    Donor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188744

    Purpose

    Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188745

    Purpose

    Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    nonTATAchr21

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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