We narrowed to 5,055 results for: cre encoding plasmid

1. AAV8-hSyn-flex-miR30-eGFP-shNts

   Plasmid

   #132717

   Purpose

   Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Nts gene which encodes neurotensin

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   GFP-shNts (Nts Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin 1

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-DIO-ASAP3Kv-WPRE

   Plasmid

   #132330

   Purpose

   expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3KV
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HCP1

   Plasmid

   #166103

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSc1-puro

   Plasmid

   #80438

   Purpose

   Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   Puromycin resistance
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6, and hPGK

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX ALIX-GFP S718/721/E

   Plasmid

   #138584

   Purpose

   Expresses fluorescent ALIX S718/721E in mammalian cells

   Depositor

   Wesley Sundquist

   Insert

   ALIX (PDCD6IP Human)
   

   Use

   Lentiviral

   Tags

   GFP and h30

   Expression

   Mammalian

   Mutation

   changed serines 718 and 721 to glutamic acids

   Promoter

   TREtight

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAcGFP-N1_CBPdIDR7-H1

   Plasmid

   #221930

   Purpose

   Transient expression of GFP-tagged CBPdeltaIDR7-H1 in mammalian cells

   Depositor

   Daniel Bose

   Insert

   CBPdeltaIDR7-H1 (CREBBP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deletion of IDR7, CBP aa 2187 - 2216

   Promoter

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcGFP-N1_CBPdIDR6-H2

   Plasmid

   #221929

   Purpose

   Transient expression of GFP-tagged CBPdeltaIDR6-H2 in mammalian cells

   Depositor

   Daniel Bose

   Insert

   CBPdeltaIDR6-H2 (CREBBP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deletion of IDR6, CBP aa 1951 - 1978

   Promoter

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMta2-EosHalo-TAFiP

   Plasmid

   #140959

   Purpose

   Targeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusion

   Depositor

   Brian Hendrich

   Insert

   metastasis-associated gene family, member 2 (Mta2 Mouse, Synthetic)
   

   Use

   Mouse Targeting

   Tags

   3xFLAG, Avi, Eos, Halo, and TEV

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRetroX-TRE3G_CBP-GFP

    Plasmid

    #221926

    Purpose

    Dox-inducible expression of CBP-GFP in mammalian cells by retroviral transduction

    Depositor

    Daniel Bose

    Insert

    CBP (CREBBP Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    insertion of CBP-GFP

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcGFP-N1_CBPdCFID

    Plasmid

    #221909

    Purpose

    Transient expression of GFP-tagged CBPdeltaCFID in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBPdeltaCFID (CREBBP Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of the CBP-FUS interaction domain (CFID)…

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MLM3636

    Plasmid

    #43860

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

    Depositor

    Keith Joung

    Insert

    None
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST26-his-hSFRP1

    Plasmid

    #189539

    Purpose

    Protein expression of SFRP1 in mammalian cells.

    Depositor

    Haian Fu

    Insert

    secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG.LSL.EGFP

    Plasmid

    #100047

    Purpose

    AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.LSL.tdTomato

    Plasmid

    #100048

    Purpose

    AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdtomato
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

    Plasmid

    #125713

    Purpose

    Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Has Service

    AAV1 and AAV5

    Insert

    eOPN3
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 331-429

    Promoter

    hSyn1

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE

    Plasmid

    #104492

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

    Insert

    jGCaMP7f
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE

    Plasmid

    #104496

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, and AAV1

    Insert

    jGCaMP7f
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hsyn-flex-Voltron-ST

    Plasmid

    #119036

    Purpose

    Cre-dependant expression of soma-localized Voltron in neurons

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Has Service

    AAV1

    Insert

    Voltron-ST
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hsyn

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE

    Plasmid

    #104493

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV Retrograde and AAV1

    Insert

    jGCaMP7b
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge